| Apoptosis of human primary B lymphocytes is inhibited by N-acetyl-L-cysteine. | |
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MedLine Citation:
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PMID: 14726501 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thiols are important molecules to control apoptosis. This study examined the effect of N-acetyl-L-cysteine (NAC) on in vitro spontaneous apoptosis of human tonsillar B lymphocytes (TBL). Results show that NAC inhibits TBL apoptosis and maintains their survival in vitro. The antiapoptotic action of NAC is progressively reduced when its addition to culture is delayed, is reversible, and is not blocked by cycloheximide. The antiapoptotic activity of NAC is associated with its ability to inhibit caspase-3 and -7 proteolytic processing, DNA-fragmentation factor 45 cleavage, and DNA fragmentation. Furthermore, NAC inhibits BID cleavage and cytochrome c release from mitochondria and increases the expression of Bcl-2 and Bcl(XL) survival proteins. However, it has no effect on caspase-9 cleavage and increases that of caspase-8 and poly(adenosine 5'-diphosphate-ribose)polymerase. We conclude that NAC-induced inhibition of TBL apoptosis is associated with inhibition of caspase-3 and -7 processing and is accompanied by changes in several regulatory components of the apoptotic process. These results pose the question of whether microenvironment thiols may in part contribute to in vivo B cell survival. |
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Authors:
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Emanuela Rosati; Rita Sabatini; Emira Ayroldi; Antonio Tabilio; Andrea Bartoli; Stefano Bruscoli; Costantino Simoncelli; Ruggero Rossi; Pierfrancesco Marconi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-04-01 |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 76 ISSN: 0741-5400 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2004 Jul |
Date Detail:
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Created Date: 2004-06-30 Completed Date: 2004-08-03 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 152-61 Citation Subset: IM |
Affiliation:
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General Pathology and Immunology Section, University of Perugia, Via Brunamonti, General Hospital-Monteluce 06100 Perugia, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology* Antiviral Agents / pharmacology* Apoptosis / drug effects*, physiology B-Lymphocytes / drug effects*, immunology Blotting, Western Caspase 3 Caspase 7 Caspases / drug effects, metabolism Cells, Cultured Cytochromes c / drug effects, secretion Humans Palatine Tonsil / cytology, immunology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antiviral Agents; 616-91-1/Acetylcysteine; 9007-43-6/Cytochromes c; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/CASP7 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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