| Apoptosis detection in brain using low-magnification dark-field microscopy. | |
| | |
MedLine Citation:
|
PMID: 10448439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Apoptosis or programmed cell death is a feature of normal brain development and a response to brain injury. Cells undergoing apoptosis have a characteristic morphology that normally can only be appreciated at high magnification. Using dark-field transmitted light microscopy to examine Nissl-stained material, we detected groups of apoptotic cells at much lower magnifications than often were required in the two injury models we tested. This method was useful for screening entire brain sections to assess regional and global patterns of injury. We predict that this technique in which we detect the clumped chromatin associated with apoptosis can be applied to other types of tissue. |
| | |
Authors:
|
M S Lange; M V Johnston; E E Tseng; W A Baumgartner; M E Blue |
Related Documents
:
|
20970339 - Meiotic errors activate checkpoints that improve gamete quality without triggering apop... 15578429 - Changes in extranucleolar transcription during actinomycin d-induced apoptosis. 15798019 - Porcine embryo development and fragmentation and their relation to apoptotic markers: a... 9205079 - Apoptosis in the subependyma of young adult rats after single and fractionated doses of... 10946849 - Nifedipine induces apoptosis in cultured vascular smooth muscle cells from spontaneousl... 21383899 - Real-world exposure of airborne particulate matter triggers oxidative stress in an anim... 20937639 - Apigenin induces apoptosis in human lung cancer h460 cells through caspase- and mitocho... 9704229 - Growth factors and the regulation of fetal growth. 9444999 - Induction of neutralizing antibodies to t-cell line-adapted and primary human immunodef... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Experimental neurology Volume: 158 ISSN: 0014-4886 ISO Abbreviation: Exp. Neurol. Publication Date: 1999 Jul |
Date Detail:
|
Created Date: 1999-09-09 Completed Date: 1999-09-09 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370712 Medline TA: Exp Neurol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 254-60 Citation Subset: IM |
Affiliation:
|
Kennedy Krieger Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Apoptosis* / drug effects, genetics, physiology Dentate Gyrus / drug effects, pathology* Dogs Hippocampus / drug effects, pathology Male Microscopy / methods Neurons / drug effects, pathology* Nissl Bodies / pathology |
| Grant Support | |
ID/Acronym/Agency:
|
NS28208/NS/NINDS NIH HHS; NS31238/NS/NINDS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hypothermia ameliorates ischemic brain damage and suppresses the release of extracellular amino acid...
Next Document: Schizophrenia--a disorder of the corollary discharge systems that integrate the motor systems of tho...