Document Detail

Apoptosis detection in brain using low-magnification dark-field microscopy.
MedLine Citation:
PMID:  10448439     Owner:  NLM     Status:  MEDLINE    
Apoptosis or programmed cell death is a feature of normal brain development and a response to brain injury. Cells undergoing apoptosis have a characteristic morphology that normally can only be appreciated at high magnification. Using dark-field transmitted light microscopy to examine Nissl-stained material, we detected groups of apoptotic cells at much lower magnifications than often were required in the two injury models we tested. This method was useful for screening entire brain sections to assess regional and global patterns of injury. We predict that this technique in which we detect the clumped chromatin associated with apoptosis can be applied to other types of tissue.
M S Lange; M V Johnston; E E Tseng; W A Baumgartner; M E Blue
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental neurology     Volume:  158     ISSN:  0014-4886     ISO Abbreviation:  Exp. Neurol.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-09-09     Completed Date:  1999-09-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  254-60     Citation Subset:  IM    
Kennedy Krieger Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
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MeSH Terms
Animals, Newborn
Apoptosis* / drug effects,  genetics,  physiology
Dentate Gyrus / drug effects,  pathology*
Hippocampus / drug effects,  pathology
Microscopy / methods
Neurons / drug effects,  pathology*
Nissl Bodies / pathology
Grant Support

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