Document Detail

Apoptosis of cultured neuroblastoma cells is induced by ceramide and not by ligation of the Fas/Apo-1/CD95 receptor.
MedLine Citation:
PMID:  10394009     Owner:  NLM     Status:  MEDLINE    
The expression of the Fas/APO-1/CD95 receptor on the neuroblastoma cell lines IMR-32, Kelly, SK-N-SH, LS and SiMa was investigated. The induction of apoptosis was attempted by incubation with Fas antibody IgM and IgG, Fas ligand and with ceramide. All neuroblastoma cell lines proved to be positive for Fas/APO-1/CD95 receptor expression by FACS. However, propidium iodide staining in FACS showed that neither incubation with the Fas antibody nor with the Fas ligand resulted in convincing apoptosis of the neuroblastoma cells. On the other hand, incubation with ceramide rapidly led to all signs of apoptosis morphologically and in the FACS. Therefore, other pathways than Fas/Apo-1/CD95 ligation must be of significance for the apoptosis of these neuroblastoma cell lines.
W Barthlen; J Schäfer; R Girgert; M Schuster; P Schweizer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemotherapy     Volume:  45     ISSN:  0009-3157     ISO Abbreviation:  Chemotherapy     Publication Date:    1999 Jul-Aug
Date Detail:
Created Date:  1999-09-09     Completed Date:  1999-09-09     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0144731     Medline TA:  Chemotherapy     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  258-67     Citation Subset:  IM    
Department of Pediatric Surgery, Eberhard Karls University Tübingen, Germany.
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MeSH Terms
Antigens, CD95 / immunology*
Apoptosis / drug effects*
Ceramides / pharmacology*
Neuroblastoma / drug therapy,  immunology,  pathology*
Signal Transduction / physiology
Tumor Cells, Cultured
Reg. No./Substance:
0/Antigens, CD95; 0/Ceramides; 0/Ligands

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