Document Detail

Apoptosis contributes to vascular lumen formation and vascular branching in human placental vasculogenesis.
MedLine Citation:
PMID:  15564598     Owner:  NLM     Status:  MEDLINE    
Placental vasculogenesis consists of several stages, including appearance of hemangioblasts and angiogenic cell islands, setting up a primitive vascular network, and transition from vasculogenesis to sprouting and nonsprouting angiogenesis. In the present study, we hypothesized that placental vasculogenesis and angiogenesis require apoptosis during the formation of primitive vascular pattern, vessel elongation, and angiogenic branching. Vasculogenesis and apoptotic cells were identified using CD31 immunohistochemistry, hematoxylin-eosin (H-E) staining, CD31-TUNEL double-labeling, and transmission-electron microscopy (TEM). No TUNEL-positive cell was detected in angiogenic cell islands; however, several TUNEL-positive cells were observed during the primitive lumen formation. Interestingly, some of the stromal cells located between vasculogenic areas during the endothelial tube elongation and angiogenic branching also were TUNEL-positive. The presence of morphological aspects of apoptosis, such as nuclear shrinkage and nuclear bodies (apoptotic bodies), also was confirmed in H-E-stained and TEM-depicted sections. Quantitative analysis showed that higher ratios for apoptotic cells were found in the core stroma of villi among the vascular branching areas and in the primitive capillary lumen compared to angiogenic cell cords and vasculatures with advanced lumens (P < 0.05). In conclusion, our results suggest that apoptosis likely is involved in the physiologic mechanisms of placental vasculogenesis and angiogenesis, such as lumen formation and angiogenic branching.
Fatma Tertemiz; Umit A Kayisli; Aydin Arici; Ramazan Demir
Related Documents :
15361838 - Recombinant cd44-habd is a novel and potent direct angiogenesis inhibitor enforcing end...
12207578 - Recent advances in cutaneous angiogenesis.
15108118 - Increased ovarian follicular angiogenesis and dynamic changes of follicular vascular pl...
18772498 - Ninein is expressed in the cytoplasm of angiogenic tip-cells and regulates tubular morp...
16329468 - Sesamol induces nitric oxide release from human umbilical vein endothelial cells.
18543288 - The binding of heparin to the extracellular matrix of endothelial cells up-regulates th...
Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-24
Journal Detail:
Title:  Biology of reproduction     Volume:  72     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-24     Completed Date:  2005-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  727-35     Citation Subset:  IM    
Department of Histology and Embryology Faculty of Medicine, Akdeniz University, Antalya 07070, Turkey.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, CD31 / metabolism
Apoptosis / physiology*
Cell Differentiation / physiology*
Endothelial Cells / cytology
Endothelium, Vascular / cytology,  physiology*
In Situ Nick-End Labeling
Neovascularization, Physiologic / physiology*
Placenta / blood supply*,  cytology,  physiology
Pregnancy Trimester, First / physiology
Reference Values
Reg. No./Substance:
0/Antigens, CD31

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Expression of estrogen receptors-alpha and -beta in the pregnant ovine uterine artery endothelial ce...
Next Document:  Molecular characterization of equine P-selectin (CD62P) and its regulation in ovarian follicles duri...