Document Detail


Apoptosis contributes to vascular lumen formation and vascular branching in human placental vasculogenesis.
MedLine Citation:
PMID:  15564598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Placental vasculogenesis consists of several stages, including appearance of hemangioblasts and angiogenic cell islands, setting up a primitive vascular network, and transition from vasculogenesis to sprouting and nonsprouting angiogenesis. In the present study, we hypothesized that placental vasculogenesis and angiogenesis require apoptosis during the formation of primitive vascular pattern, vessel elongation, and angiogenic branching. Vasculogenesis and apoptotic cells were identified using CD31 immunohistochemistry, hematoxylin-eosin (H-E) staining, CD31-TUNEL double-labeling, and transmission-electron microscopy (TEM). No TUNEL-positive cell was detected in angiogenic cell islands; however, several TUNEL-positive cells were observed during the primitive lumen formation. Interestingly, some of the stromal cells located between vasculogenic areas during the endothelial tube elongation and angiogenic branching also were TUNEL-positive. The presence of morphological aspects of apoptosis, such as nuclear shrinkage and nuclear bodies (apoptotic bodies), also was confirmed in H-E-stained and TEM-depicted sections. Quantitative analysis showed that higher ratios for apoptotic cells were found in the core stroma of villi among the vascular branching areas and in the primitive capillary lumen compared to angiogenic cell cords and vasculatures with advanced lumens (P < 0.05). In conclusion, our results suggest that apoptosis likely is involved in the physiologic mechanisms of placental vasculogenesis and angiogenesis, such as lumen formation and angiogenic branching.
Authors:
Fatma Tertemiz; Umit A Kayisli; Aydin Arici; Ramazan Demir
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-11-24
Journal Detail:
Title:  Biology of reproduction     Volume:  72     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-24     Completed Date:  2005-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  727-35     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology Faculty of Medicine, Akdeniz University, Antalya 07070, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD31 / metabolism
Apoptosis / physiology*
Cell Differentiation / physiology*
Endothelial Cells / cytology
Endothelium, Vascular / cytology,  physiology*
Female
Humans
In Situ Nick-End Labeling
Neovascularization, Physiologic / physiology*
Placenta / blood supply*,  cytology,  physiology
Pregnancy
Pregnancy Trimester, First / physiology
Reference Values
Chemical
Reg. No./Substance:
0/Antigens, CD31

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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