Document Detail


Apoptosis and cell cycle arrest of human colorectal cancer cell line HT-29 induced by vanillin.
MedLine Citation:
PMID:  19679064     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Vanillin is responsible for the flavor and smell of vanilla, a widely used flavoring agent. Previous studies showed that vanillin could enhance the repair of mutations and thus function as an anti-mutagen. However, its role in cancer, a disease that is closely related to mutation has not yet been fully elucidated.
METHODS: Hence, this study investigated the cytolytic and cytostatic properties of vanillin against HT-29, a human colorectal cancer cell line. Methods used including cell viability assay, acridine orange (AO)-ethidium bromide (EB) double staining cell morphological analysis, Cell cycle analysis, annexin V-propidium iodide apoptosis test and 5-bromo-2-deoxyuridine (BrdU)-labeling cell proliferation assay.
RESULTS: Results showed that apoptosis was induced by vanillin and the IC(50) for HT-29 and NIH/3T3 normal cell lines were 400 microg/ml and 1000 microg/ml, respectively. Different concentrations of vanillin arrest cell cycle at different checkpoints. 5-Bromo-2-deoxyuridine-labeling cell proliferation assay showed that G0/G1 arrest was achieved at lower concentration of vanillin (200 microg/ml) while cell cycle analysis by flow cytometer showed that G2/M arrest occurs at higher concentration of vanillin (1000 microg/ml).
CONCLUSION: Cytolytic and cytostatic effects shown by vanillin showed that it could be a useful colorectal cancer preventive agent. Further in vivo study should be carried out to confirm that similar effects could happen in animals.
Authors:
Ketli Ho; Latifah Saiful Yazan; Norsharina Ismail; Maznah Ismail
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-03
Journal Detail:
Title:  Cancer epidemiology     Volume:  33     ISSN:  1877-783X     ISO Abbreviation:  Cancer Epidemiol     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-14     Completed Date:  2009-10-20     Revised Date:  2011-06-17    
Medline Journal Info:
Nlm Unique ID:  101508793     Medline TA:  Cancer Epidemiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  155-60     Citation Subset:  IM    
Affiliation:
Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antimutagenic Agents / pharmacology*
Apoptosis / drug effects*
Benzaldehydes / pharmacology*
Cell Cycle / drug effects*
Cell Proliferation / drug effects*
Cells, Cultured
Colorectal Neoplasms / drug therapy*,  pathology*
Flow Cytometry
HT29 Cells
Humans
Mice
NIH 3T3 Cells
Chemical
Reg. No./Substance:
0/Antimutagenic Agents; 0/Benzaldehydes; 121-33-5/vanillin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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