| Apoptosis-associated cleavage of beta-catenin in human colon cancer and rat hepatoma cells. | |
| | |
MedLine Citation:
|
PMID: 10224675 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Proteases belonging to the caspase family play a crucial role in apoptotic processes. Identification of protein cleavage specific to apoptosis may therefore provide further information about the mechanisms of apoptosis. In this study, apoptosis and necrosis were induced in cells of the human colon cancer cell lines, WiDr and DLD-1, and the resulting protein cleavage patterns investigated for beta-catenin. beta-Catenin was detected as a 92 kDa protein in control viable cells, while 65-72 kDa beta-catenin cleavage fragments were characteristically observed in apoptotic cells. These fragments were not observed in necrotic cell death. Similar apoptosis-specific beta-catenin cleavage was also demonstrated in the rat hepatoma cell line McA-RH7777, suggesting that the beta-catenin cleavage is a common event in apoptosis in various cell types. The formation of 65-72 kDa beta-catenin cleavage fragments was completely prevented by a caspase-1 inhibitor Z-VAD-CH2F and a caspase-3 inhibitor Z-DEVD-CH2F, indicating that the cleavage is associated with caspase-dependent process. Since beta-catenin is implicated in cell adhesion and signal transduction, these findings may suggest various possible roles of beta-catenin degradation in the dramatic cytoskeletal and morphological changes, as well as signaling events that accompany apoptosis. |
| | |
Authors:
|
K Fukuda |
Related Documents
:
|
11098135 - Zinc induces mixed types of cell death, necrosis, and apoptosis, in molt-4 cells. 9770325 - Activation of interleukin-1beta-converting enzyme by nigericin is independent of apopto... 13130505 - Cystamine inhibits transglutaminase and caspase-3 cleavage in glutamate-exposed astrogl... 8853905 - Bcl-xl overexpression inhibits taxol-induced yama protease activity and apoptosis. 17673665 - Negative regulation of toll-like receptor signaling by nf-kappab p50 ubiquitination blo... 16537615 - Measles virus targets dc-sign to enhance dendritic cell infection. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The international journal of biochemistry & cell biology Volume: 31 ISSN: 1357-2725 ISO Abbreviation: Int. J. Biochem. Cell Biol. Publication Date: 1999 Mar-Apr |
Date Detail:
|
Created Date: 1999-06-08 Completed Date: 1999-06-08 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 9508482 Medline TA: Int J Biochem Cell Biol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 519-29 Citation Subset: IM |
Affiliation:
|
Department of Oriental Medicine, Gifu University School of Medicine, Japan. kfukuda@cc.gifu-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actins
/
metabolism Animals Apoptosis* Butyrates / pharmacology Cadherins / metabolism Cells, Cultured Colonic Neoplasms / metabolism* Cytoskeletal Proteins / metabolism* DNA Fragmentation Electrophoresis, Polyacrylamide Gel Humans Hydroxamic Acids / pharmacology Immunoblotting Liver Neoplasms, Experimental / metabolism* Necrosis Protease Inhibitors / metabolism Rats Time Factors Trans-Activators* beta Catenin |
| Chemical | |
Reg. No./Substance:
|
0/Actins; 0/Butyrates; 0/CTNNB1 protein, human; 0/Cadherins; 0/Catnb protein, rat; 0/Cytoskeletal Proteins; 0/Hydroxamic Acids; 0/Protease Inhibitors; 0/Trans-Activators; 0/beta Catenin; 58880-19-6/trichostatin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Overexpression of sense or antisense human gastrin mRNA does not affect proliferation of normal rat ...
Next Document: Charge derivatization of peptides for analysis by mass spectrometry.