Document Detail


Apoptosis of androgen-independent mammary and prostate cell lines induced by topoisomerase inhibitors: common pathway of gene regulation.
MedLine Citation:
PMID:  9216669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
New treatments for hormone-independent tumor are urgently needed since androgen-dependent cancer cells eventually progress to -independent cells after hormonal manipulation. In the present study, etoposide and camptothecin were used to induce proliferation-dependent death of these cells. Each of the agents, at the doses used, induces the apoptosis of AT-3, CS 2, and TSU-pr1 cells based upon the temporal sequence of DNA fragmentation, morphologic changes and loss of cell viability. Northern blot analysis was used to identify a series of genes whose expression is enhanced during the apoptotic pathway. During the apoptotic process induced by the agents, expression of cyclin-dependent kinase inhibitor p27 increased. Flow cytometric analysis showed that the treatment resulted in a block in G2/M of the cell cycle. These results demonstrate that these cells retain the ability to undergo apoptosis by etoposide and camptothecin, and cyclin-dependent kinase inhibitor plays some role during apoptotic pathway.
Authors:
Y Furuya; S Ohta; H Ito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  17     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1997 May-Jun
Date Detail:
Created Date:  1997-08-04     Completed Date:  1997-08-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  2089-93     Citation Subset:  IM    
Affiliation:
Department of Urology, School of Medicine, Chiba University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Androgens / pharmacology*
Animals
Apoptosis / drug effects*
Camptothecin / toxicity*
Cell Cycle / drug effects*
Cell Cycle Proteins*
Cell Survival / drug effects
Cyclin-Dependent Kinase Inhibitor p27
Cyclin-Dependent Kinases / antagonists & inhibitors
DNA Fragmentation
DNA Topoisomerases, Type I / antagonists & inhibitors*
DNA Topoisomerases, Type II / antagonists & inhibitors*
Enzyme Inhibitors / toxicity*
Etoposide / toxicity*
Female
Humans
Male
Mammary Neoplasms, Experimental
Mice
Microtubule-Associated Proteins / biosynthesis
Prostatic Neoplasms
Rats
Tumor Cells, Cultured
Tumor Suppressor Proteins*
Chemical
Reg. No./Substance:
0/Androgens; 0/Cdkn1b protein, mouse; 0/Cdkn1b protein, rat; 0/Cell Cycle Proteins; 0/Enzyme Inhibitors; 0/Microtubule-Associated Proteins; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 33419-42-0/Etoposide; 7689-03-4/Camptothecin; EC 2.7.11.22/Cyclin-Dependent Kinases; EC 5.99.1.2/DNA Topoisomerases, Type I; EC 5.99.1.3/DNA Topoisomerases, Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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