| Apoptosis and reduced cell proliferation of HL-60 cell line caused by human telomerase reverse transcriptase inhibition by siRNA. | |
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MedLine Citation:
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PMID: 20628241 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The close correlation between telomerase activity and human telomerase reverse transcriptase (hTERT) expression has made hTERT to be considered as a selective molecular target for human cancer therapy. In this study, the ability of short-interfering RNA (siRNA) to downregulate hTERT expression and its correlation with cell growth and apoptosis in the promyelocytic cell line HL-60 was evaluated. MATERIALS AND METHODS: hTERT siRNA was designed and transfected to HL-60. hTERT mRNA expression, cell proliferation and apoptotic cells were measured. RESULTS AND CONCLUSION: The results indicated that hTERT siRNA resulted in 97.2 ± 0.6% downregulation of the hTERT mRNA content; inhibition of the cell proliferation rate was about 52.8 ± 2.3% and the apoptotic index of cells was 30.5 ± 1.5%. hTERT plays an essential role in cell proliferation and control of the viability of leukemic cells, thus promising the development of drugs for leukemia. |
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Authors:
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Ebrahim Miri-Moghaddam; Abdolkhaleg Deezagi; Zahra Sohaila Soheili; Parvin Shariati |
Publication Detail:
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Type: Journal Article Date: 2010-07-14 |
Journal Detail:
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Title: Acta haematologica Volume: 124 ISSN: 1421-9662 ISO Abbreviation: Acta Haematol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-10-07 Completed Date: 2010-10-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0141053 Medline TA: Acta Haematol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 72-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 S. Karger AG, Basel. |
Affiliation:
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Department of Hematology-Immunology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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physiology* Cell Division / physiology Enzyme Activation / genetics Gene Therapy / methods* HL-60 Cells Humans Leukemia, Promyelocytic, Acute / therapy* RNA, Messenger / metabolism RNA, Small Interfering / pharmacology* Telomerase* / antagonists & inhibitors, genetics, metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/RNA, Small Interfering; EC 2.7.7.49/TERT protein, human; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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