Document Detail


Apoptosis of Hela cells induced by extract from Cremanthodium humile.
MedLine Citation:
PMID:  17597278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cremanthodium humile (C. humile) is a traditional herbal medicine for treatment of inflammation. Based on initial screening results, the purpose of this study was to evaluate the cytotoxic effect on four human cancer cell lines and one non-cancer cell line (293), then to determine the possible mechanisms of cell death elicited by the extract of C. humile on Hela cells. We have found the ether extract of C. humile (CH-EE) strongly decreased the survival rate of the four human tumor cell lines: Hela, A549, HepG2 and SW480. The cytotoxic effect of CH-EE on 293 was smaller than on tumor cell lines. Flow cytometry assays and nuclear staining showed that CH-EE induced apoptosis in Hela cells. This process was accompanied by the collapse of mitochondrial membrane potential, the release of cytochrome c and the activation of caspase-3/7 and -9. Furthermore, CH-EE generated reactive oxygen species (ROS) in Hela cells. These results indicate that CH-EE induces apoptosis in Hela cells through a ROS-mediated mitochondrial dysfunction pathway.
Authors:
Hui Li; Li-Juan Wang; Guo-Fu Qiu; Jian-Qing Yu; Shu-Cai Liang; Xian-Ming Hu
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Publication Detail:
Type:  Journal Article     Date:  2007-05-13
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  45     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-08-16     Completed Date:  2007-10-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2040-6     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Virology, College of Pharmacy, Wuhan University, Wuhan 430072, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Asteraceae / chemistry*
Blotting, Western
Caspases / metabolism
Cell Cycle / drug effects
Cell Survival / drug effects
Chromatography, High Pressure Liquid
Cytochromes c / metabolism
DNA Fingerprinting
Hela Cells
Humans
Indicators and Reagents
Membrane Potentials
Microscopy, Fluorescence
Mitochondria / drug effects
Plant Extracts / pharmacology
Reactive Oxygen Species / metabolism
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Plant Extracts; 0/Reactive Oxygen Species; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspases

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