Document Detail

Apoptin enhances radiation-induced cell death in poorly responding head and neck squamous cell carcinoma cells.
MedLine Citation:
PMID:  19874285     Owner:  NLM     Status:  MEDLINE    
Treatment of head and neck cancers is still rather poor and worldwide new treatment options are sought. Sensitizing radioresistant tumours by combining irradiation with other therapeutics to induce apoptosis are widely investigated. We examined whether chicken anaemia virus-derived apoptin protein would have a beneficial effect on irradiation of radiosensitive SCC61 and radioresistant SQD9 human head and neck squamous carcinoma cell lines. In both cell lines, concurrent exposure to irradiation and apoptin resulted in analysed mitochondrial cytochrome c release and in cleavage of caspase-3, whereas irradiation alone of SQD9 cells under identical conditions did not. Moreover, in comparison with the irradiation, only the synchronized treatment of apoptin and irradiation resulted in increased cell death in especially the radioresistant SQD9 cells, as measured by means of a colony survival assay. Our data reveal that apoptin treatment represents an effective way for enhancing radiotherapy of tumours responding poorly to radiotherapy.
Remilio A L Schoop; Elizabeth M E Verdegaal; Robert J Baatenburg de Jong; Mathieu H M Noteborn
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Publication Detail:
Type:  Journal Article     Date:  2009-10-27
Journal Detail:
Title:  Basic & clinical pharmacology & toxicology     Volume:  106     ISSN:  1742-7843     ISO Abbreviation:  Basic Clin. Pharmacol. Toxicol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-18     Completed Date:  2010-02-23     Revised Date:  2010-10-29    
Medline Journal Info:
Nlm Unique ID:  101208422     Medline TA:  Basic Clin Pharmacol Toxicol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  130-4     Citation Subset:  IM    
Department of Otolaryngology, Head and Neck Surgery, Leiden University Medical Center, Leiden, the Netherlands.
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MeSH Terms
Capsid Proteins / pharmacology*
Carcinoma, Squamous Cell / drug therapy*,  pathology,  radiotherapy
Caspase 3 / drug effects,  metabolism,  radiation effects
Cell Death / drug effects,  radiation effects
Cell Line, Tumor
Combined Modality Therapy
Cytochromes c / drug effects,  metabolism,  radiation effects
Head and Neck Neoplasms / drug therapy*,  pathology,  radiotherapy
Mitochondria / drug effects,  metabolism,  radiation effects
Radiation-Sensitizing Agents / pharmacology*
Reg. No./Substance:
0/Capsid Proteins; 0/Radiation-Sensitizing Agents; 0/VP3 protein, Chicken anemia virus; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspase 3

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