| Apolipoprotein(a) inhibits lipopolysaccharide-induced IL-6 secretion in human astrocytoma cell line by interfering with lipopolysaccharide signaling. | |
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MedLine Citation:
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PMID: 21042834 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To examine the role of lipoprotein(a) [Lp(a)] on the inflammatory response of cells in the nervous system by investigating its effect on lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) secretion. MATERIALS AND METHODS: Human astrocytoma U373 cells were treated with recombinant apolipoprotein(a) [r-apo(a)] A10K (175-11 nM), alone or in combination with LPS (100 and 10 ng/ml). IL-6 levels were evaluated by immunoblotting. Statistical analysis was performed by one-way ANOVA. RESULTS: r-apo(a) caused dose-dependent inhibition of LPS-induced IL-6 secretion (100 ng/ml LPS, p = 0.0205; 10 ng/ml LPS, p = 0.0005). Pre-treatment of cells with 88 nM r-apo(a), rinsing, and activation with 10 ng/ml LPS did not reverse the inhibition (p = 0.0048), which could be reversed by supplementation with excess serum (5-20%) (p = 0.0454) or recombinant CD14 (2.0-0.05 μg/ml) (p = 0.0230). CONCLUSIONS: Our data indicate that apo(a) plays a natural anti-endotoxin role which relies on its interference with cell-associated and serum components of LPS signaling. |
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Authors:
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Guglielmina Chimienti; Anna Mezzapesa; Grazia M Liuzzi; Tiziana Latronico; Gabriella Pepe |
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Publication Detail:
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Type: Journal Article Date: 2010-11-02 |
Journal Detail:
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Title: Inflammation research : official journal of the European Histamine Research Society ... [et al.] Volume: 60 ISSN: 1420-908X ISO Abbreviation: Inflamm. Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-16 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508160 Medline TA: Inflamm Res Country: Switzerland |
Other Details:
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Languages: eng Pagination: 329-35 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, "Ernesto Quagliariello", University of Bari, Via Orabona 4, 70126, Bari, Italy. |
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