Document Detail


The apolipoprotein A-I level is downregulated in the granulosa cells of patients with polycystic ovary syndrome and affects steroidogenesis.
MedLine Citation:
PMID:  20426491     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder found in women. The etiology of PCOS is still not clear, and there are no available studies on the proteome analysis of granulosa cells (GCs) in PCOS patients. To identify the pathogenic mechanisms and potential diagnostic markers for PCOS, we conducted proteomic profiling of GCs in PCOS patients by two-dimensional gel electrophoresis and liquid chromatography coupled with mass spectrometry (LC-MS/MS) analyses. The proteomic analysis yielded eight downregulated and 12 upregulated proteins in PCOS patients, among which apolipoprotein A-I (ApoA-I) showed significant downregulation in PCOS patients as confirmed by Western blotting. Knockdown of ApoA-I decreased the number of transcripts of steroidogenic enzymes in a granulosa cell line (KGN), while its overexpression generally increased the level of expression of these enzymes. Furthermore, modulation of the expression level of ApoA-I in the granulosa cells altered progesterone production. Therefore, this study suggests that ApoA-I can be useful as a granulosa cell biomarker of PCOS patients and that downregulated ApoA-I may be related to the disturbed production of steroid hormones in PCOS patients.
Authors:
Dong-Hee Choi; Woo-Sik Lee; Miae Won; Mira Park; Ho-Oak Park; Eunju Kim; Kyoung-Ah Lee; Jeehyeon Bae
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of proteome research     Volume:  9     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-03     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4329-36     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Bundang CHA Women's Hospital, and Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea 463-836.
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MeSH Terms
Descriptor/Qualifier:
Adult
Apolipoprotein A-I / genetics*,  metabolism*
Blotting, Western
Cell Line
Chromatography, Liquid
Down-Regulation
Electrophoresis, Gel, Two-Dimensional
Female
Gene Expression Regulation
Gene Knockdown Techniques
Granulosa Cells / metabolism*
Humans
Polycystic Ovary Syndrome / genetics,  metabolism*,  pathology
Progesterone / biosynthesis*,  metabolism
Proteome / chemistry,  metabolism
Proteomics / methods
Reproducibility of Results
Tandem Mass Spectrometry
Chemical
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Proteome; 57-83-0/Progesterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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