| Apolipoprotein E2/E3/E4 genotyping with agarose gels. | |
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MedLine Citation:
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PMID: 7813084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here we report an improved, simple method to assign the human apolipoprotein (apo) E genotype and its isoforms, apo E2, apo E3, and apo E4. Genomic DNA was amplified with specific primers that included the polymorphic region of amino acids 112 and 158. Digestion of the product with Hhal resulted in unique fragments that were separated on Meta-Phor agarose instead of polyacrylamide. The pattern of unique DNA fragments obtained unequivocally characterizes the different apo E alleles. |
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Authors:
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G M Dallinga-Thie; M van Linde-Sibenius Trip; L A Kock; T W De Bruin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry Volume: 41 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-02-09 Completed Date: 1995-02-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 73-5 Citation Subset: IM |
Affiliation:
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Department of Medicine and Endocrinology, Utrecht University, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apolipoprotein E2 Apolipoprotein E3 Apolipoprotein E4 Apolipoproteins E / genetics* Base Sequence Electrophoresis, Agar Gel / methods* Genotype* Humans Isoelectric Focusing Molecular Sequence Data Phenotype Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein E2; 0/Apolipoprotein E3; 0/Apolipoprotein E4; 0/Apolipoproteins E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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