| Apolipoprotein A-V modulates insulin secretion in pancreatic beta-cells through its interaction with midkine. | |
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MedLine Citation:
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PMID: 19910685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apolipoprotein A-V is an important determinant of plasma triglyceride level in both humans and mice. This study showed the physiological impact of apoA-V on insulin secretion in rat pancreatic beta-cells (INS-1 cells). In order to precise the mechanism of action, binding experiments coupled to mass spectrometry were performed to identify a potential membrane receptor. Results showed an interaction between apoA-V and midkine protein. Confocal microscopy confirmed the plasma membrane co-localisation of this two-proteins after the treatment of INS-1 cells with the apo-AV recombinant protein and indicated that the cell surface midkine could be involved in apoA-V endocytosis, since these two proteins were co-translocated at the plasma membrane or in the cytosol compartment. This co-localisation is correlated with an increase in insulin secretion in a dose dependant manner during short incubation period. Reduction of midkine expression by small interfering RNA duplexes revealed a decrease in the ability of these transfected cells to secrete insulin in presence of apoA-V. Competition experiments for the apoA-V-midkine binding at the cell surface using antibody directed against midkine is able to influence INS-1 cell function as insulin secretion. Our results showed apoA-V ability to enhance insulin secretion in beta-cells and provide evidence of an internalization pathway involving the midkine as partner. |
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Authors:
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Audrey Helleboid-Chapman; Maxime Nowak; Stéphane Helleboid; Emmanuelle Moitrot; Corinne Rommens; Hélène Dehondt; Laurent Héliot; Hervé Drobecq; Jamila Fruchart-Najib; Jean-Charles Fruchart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-04 |
Journal Detail:
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Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 24 ISSN: 1421-9778 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-11-13 Completed Date: 2010-02-17 Revised Date: 2011-05-06 |
Medline Journal Info:
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Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
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Languages: eng Pagination: 451-60 Citation Subset: IM |
Copyright Information:
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2009 S. Karger AG, Basel. |
Affiliation:
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Université Lille Nord de France, Inserm, UDSL and Institut Pasteur de Lille, France. audrey.helleboid@univ-lille2.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Apolipoproteins / analysis, metabolism* Cell Line, Tumor Cytokines / analysis, genetics, metabolism* Endocytosis Immunoprecipitation Insulin / secretion* Insulin-Secreting Cells / metabolism* Molecular Sequence Data Protein Binding RNA, Small Interfering / metabolism Rats Recombinant Proteins / metabolism, pharmacology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Chemical | |
Reg. No./Substance:
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0/Apoa5 protein, rat; 0/Apolipoproteins; 0/Cytokines; 0/RNA, Small Interfering; 0/Recombinant Proteins; 11061-68-0/Insulin; 137497-38-2/midkine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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