Document Detail


Apolipoprotein E distribution among different plaque types in Alzheimer's disease: implications for its role in plaque progression.
MedLine Citation:
PMID:  8875468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We sought to determine the pattern of ApoE immunoreactivity in mesial temporal lobe tissue from 12 Alzheimer patients, age 66-88, and to determine the distribution of this immunoreactivity among different plaque types representing hypothesized stages of plaque evolution. In these patients, the cortical area of ApoE immunoreactivity was 30% that of beta-amyloid. Only 6% of diffuse non-neuritic amyloid deposits were even weakly ApoE immunoreactive (ApoE+). This is in contrast to our previous demonstration that microglia overexpressing interleukin-1 (IL-1) are present in most diffuse non-neuritic deposits. Eighty-three per cent of diffuse neuritic plaques and 86% of dense-core neuritic plaques were highly ApoE+, consistent with IL-1-induced astrocyte activation and synthesis of ApoE resulting in the appearance of ApoE immunoreactivity in neuritic plaques. Dense-core non-neuritic ('burned out') plaques were only rarely (6%) ApoE+. These results, together with the known trophic and toxic effects of ApoE on neurites, suggest that plaque-associated ApoE contributes to the formation of overgrown degenerating (dystrophic) neurites in plaques. However, the fact that some neuritic plaques are not ApoE+ suggests contributions by additional trophic and toxic factors. Our results are also consistent with a role for ApoE in the condensation of diffuse amyloid deposits into a beta-pleated-sheet form that occurs concomitant with dystrophic neurite formation in the neuritic beta-amyloid plaques of Alzheimer's disease.
Authors:
J G Sheng; R E Mrak; W S Griffin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuropathology and applied neurobiology     Volume:  22     ISSN:  0305-1846     ISO Abbreviation:  Neuropathol. Appl. Neurobiol.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-01-23     Completed Date:  1997-01-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7609829     Medline TA:  Neuropathol Appl Neurobiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  334-41     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, USA.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Alzheimer Disease / metabolism*,  pathology*
Apolipoproteins E / metabolism*
Blotting, Western
Female
Humans
Image Processing, Computer-Assisted
Immunohistochemistry
Male
Neurites / ultrastructure
Temporal Lobe / metabolism*,  pathology*
Grant Support
ID/Acronym/Agency:
AG10208/AG/NIA NIH HHS; AG12411/AG/NIA NIH HHS; NS27414/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins E

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