Document Detail

Apolipoprotein C-I reduces cholesteryl esters selective uptake from LDL and HDL by binding to HepG2 cells and lipoproteins.
MedLine Citation:
PMID:  19761869     Owner:  NLM     Status:  MEDLINE    
Plasma cholesterol from low- and high-density lipoproteins (LDL and HDL) are cleared from the circulation by specific receptors that either totally degrade lipoproteins as the LDL receptor or selectively take up their cholesteryl esters (CE) like the scavenger receptor class B type I (SR-BI). The aim of the present study was to define the effect of apoC-I on the uptake of LDL and HDL(3) by HepG2 cells. In experiments conducted with exogenously added purified apoC-I, no significant effect was observed on lipoprotein-protein association and degradation; however, LDL- and HDL(3)-CE selective uptake was significantly reduced in a dose-dependent manner. This study also shows that apoC-I has the ability to associate with HepG2 cells and with LDL and HDL(3). Moreover, pre-incubation of HepG2 cells with apoC-I reduces HDL(3)-CE selective uptake and pre-incubation of LDL and HDL(3) with apoC-I decreases their CE selective uptake by HepG2 cells. Thus, apoC-I can accomplish its inhibitory effect on SR-BI activity by either binding to SR-BI or lipoproteins. We conclude that by reducing hepatic lipoprotein-CE selective uptake, apoC-I has an atherogenic character.
Veneta Krasteva; Mathieu R Brodeur; F?lix-Labont? Tremblay; Louise Falstrault; Louise Brissette
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-15
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1801     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-11-30     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  42-8     Citation Subset:  IM    
D?partement des Sciences Biologiques, Universit? du Qu?bec ? Montr?al, C.P. 8888, Succursale Centre-ville, Montr?al, Qu?bec, Canada H3C 3P8.
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MeSH Terms
Apolipoprotein C-I / metabolism*
Binding Sites
Cells, Cultured
Cholesterol Esters / metabolism*
Lipoproteins / blood,  metabolism*
Lipoproteins, HDL / blood,  metabolism*
Lipoproteins, LDL / blood,  metabolism*
Reg. No./Substance:
0/Apolipoprotein C-I; 0/Cholesterol Esters; 0/Lipoproteins; 0/Lipoproteins, HDL; 0/Lipoproteins, LDL

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