Document Detail

Apolipoprotein B production and very low density lipoprotein secretion by calf liver slices.
MedLine Citation:
PMID:  10393338     Owner:  NLM     Status:  MEDLINE    
Secretion of triglycerides by the liver in ruminants as components of very low density lipoproteins particles is low as compared with that in primates or rodents. The rate-limiting steps for the hepatic export of very low density lipoproteins have been studied in liver slices to determine the origin of the low lipotropic capacity of calf liver compared to that of rat liver. The rates of production of apolipoprotein B (apo B) and albumin as well as the rate of secretion of VLDL-apolipoproteins were measured during 12-h incubation of liver slices in organo-culture using [35S]methionine-cysteine labeling. Hepatic apo B production was similar in the two animal species but the VLDL-apolipoprotein secretion rate for calf liver slices amounted to only 20% of that observed for rat liver slices. Although calf and rat liver slices synthesized similar amounts of total protein, the hepatic production of albumin, measured in cells and media, was much higher in calf than rat liver slices (around 2.7-fold), whereas the rate secretion of albumin was similar in the two species. Our results showed that the slow rate of secretion of VLDL by calf liver cells was not consecutive to a low rate of synthesis of apo B but rather to a defect in VLDL assembly and/or secretion.
D Gruffat-Mouty; B Graulet; D Durand; M E Samson-Bouma; D Bauchart
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  126     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-10-28     Completed Date:  1999-10-28     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  188-93     Citation Subset:  IM    
Unité Métabolismes Energétique et Lipidique, Institut National de la Recherche Agronomique, Centre de Recherches Clermont Ferrand-Theix, 63122 Saint Genès Champanelle, France.
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MeSH Terms
Albumins / metabolism
Apolipoproteins B / metabolism*
Culture Media
Cysteine / metabolism
Dipeptides / metabolism
Lipoproteins, VLDL / metabolism,  secretion*
Liver / metabolism*,  secretion
Methionine / metabolism
Oleic Acid / metabolism
Rats, Sprague-Dawley
Species Specificity
Sulfur Radioisotopes
Reg. No./Substance:
0/Albumins; 0/Apolipoproteins B; 0/Culture Media; 0/Dipeptides; 0/Lipoproteins, VLDL; 0/Sulfur Radioisotopes; 112-80-1/Oleic Acid; 52-90-4/Cysteine; 63-68-3/Methionine

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