| Apocynin but not L-arginine prevents and reverses dexamethasone-induced hypertension in the rat. | |
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MedLine Citation:
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PMID: 16580579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Dexamethasone (Dex)-hypertension in rats is associated with increased oxidative stress. We investigated effects of the NAD(P)H oxidase inhibitor apocynin and the nitric oxide (NO) precursor L-arginine on Dex-hypertension to determine the relative roles of NAD(P)H oxidase and uncoupling in the reactive oxygen species (ROS) generation and hypertension. METHODS: Male Sprague-Dawley rats (n = 10/group) received Dex (20 microg/kg/day subcutaneously) or saline (vehicle) for 14 days. In a prevention study, rats received 4 days of apocynin treatement (1.5 mmol/L in drinking water) followed by Dex/saline for 12 days. In reversal studies, apocynin or L-arginine was given from day 8 to 14. Systolic blood pressure (SBP) was measured by tail cuff, and thymus weight was used as a marker of glucocorticoid activity. RESULTS: Administration of Dex increased SBP (104 +/- 3 to 122 +/- 3 mm Hg, P < .01, mean +/- SEM) and decreased thymus and body weight (P' < .05). Apocynin alone had no effect on SBP, BW, or thymus weight. Apocynin prevented (122 +/- 4 Dex, 111 +/- 3 mm Hg Apocynin+Dex, P' < .05) and reversed Dex-hypertension (130 +/- 4 to 116 +/- 4 mm Hg, P < .01). L-arginine did not reverse Dex-hypertension. CONCLUSIONS: In male SD rats, apocynin but not l-arginine prevented and reversed Dex-hypertension, suggesting that NAD(P)H oxidase-mediated superoxide production but not endothelial nitric oxide synthase uncoupling is important in Dex-hypertension. |
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Authors:
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Lexian Hu; Yi Zhang; Pek S Lim; Yuchun Miao; Chrismin Tan; Katja U S McKenzie; Christopher G Schyvens; Judith A Whitworth |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of hypertension Volume: 19 ISSN: 0895-7061 ISO Abbreviation: Am. J. Hypertens. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-03 Completed Date: 2006-08-22 Revised Date: 2009-02-24 |
Medline Journal Info:
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Nlm Unique ID: 8803676 Medline TA: Am J Hypertens Country: United States |
Other Details:
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Languages: eng Pagination: 413-8 Citation Subset: IM |
Affiliation:
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High Blood Pressure Research Unit, The John Curtin School of Medical Research, The Australian National University, Canberra, Australian Capital Territory, Australia. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetophenones
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pharmacology* Animals Arginine / deficiency, pharmacology* Blood Pressure / drug effects Dexamethasone / adverse effects* Endothelium, Vascular / metabolism Enzyme Inhibitors / pharmacology* Hypertension / chemically induced*, physiopathology, prevention & control* Male NADPH Oxidase / antagonists & inhibitors, physiology Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type III / metabolism Organ Size Oxidative Stress / drug effects, physiology Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism Superoxides / metabolism Thymus Gland / pathology |
| Chemical | |
Reg. No./Substance:
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0/Acetophenones; 0/Enzyme Inhibitors; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 498-02-2/acetovanillone; 50-02-2/Dexamethasone; 74-79-3/Arginine; EC 1.14.13.39/NOS3 protein, human; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.6.3.1/NADPH Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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