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ApoB-INDEPENDENT ENZYME IMMUNOASSAY FOR LIPOPROTEIN(a) BY CAPTURE ON IMMOBILIZED LECTIN (JACALIN).
MedLine Citation:
PMID:  23537301     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).
Authors:
Anuradha Sreekumar; Geetha Mandagini; Sabarinath P Subramanian; Appukuttan P Sankunni
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunoassay & immunochemistry     Volume:  34     ISSN:  1532-4230     ISO Abbreviation:  J Immunoassay Immunochem     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100963688     Medline TA:  J Immunoassay Immunochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  166-79     Citation Subset:  IM    
Affiliation:
a Department of Biochemistry , Sree Chitra Tirunal Institute for Medical Sciences and Technology , Thiruvananthapuram , Kerala , India.
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