Document Detail


The Aplysia mytilus inhibitory peptide-related peptides: identification, cloning, processing, distribution, and action.
MedLine Citation:
PMID:  10531464     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuropeptides are a ubiquitous class of signaling molecules. In our attempt to understand the generation of feeding behavior in Aplysia, we have sought to identify and fully characterize the neuropeptides operating in this system. Preliminary evidence indicated that Mytilus inhibitory peptide (MIP)-like peptides are present and operating in the circuitry that generates feeding in Aplysia. MIPs were originally isolated from the bivalve mollusc Mytilus edulis, and related peptides have been identified in other invertebrate species, but no precursor has been identified. In this study, we describe the isolation and characterization of novel Aplysia MIP-related peptides (AMRPs) and their precursor. Several AMRPs appear to have some structural and functional features similar to vertebrate opioid peptides. We use matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to confirm that all 14 AMRPs predicted by the precursor are processed in isolated neurons. Northern analysis, whole-mount in situ hybridization, and immunohistochemistry are used to map the abundant expression of these peptides in the CNS and peripheral tissues such as the digestive tract, vasculature, and the reproductive organs. Physiological studies demonstrate that the rank order of the inhibitory actions of these peptides is different for three target muscles. These results underscore the importance of using a multidisciplinary approach to identifying and characterizing the actions of neuropeptides in an effort to gain understanding of their role in systems of interest. The widespread distribution of the AMRPs indicates that they may be operating in many different systems of Aplysia.
Authors:
Y Fujisawa; Y Furukawa; S Ohta; T A Ellis; N C Dembrow; L Li; P D Floyd; J V Sweedler; H Minakata; K Nakamaru; F Morishita; O Matsushima; K R Weiss; F S Vilim
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  19     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-19     Completed Date:  1999-11-19     Revised Date:  2009-02-07    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9618-34     Citation Subset:  IM    
Affiliation:
Suntory Institute for Bioorganic Research, Shimamoto, Mishima, Osaka 618-8503, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aplysia
Bivalvia
Cloning, Molecular
Ganglia, Invertebrate / chemistry*,  cytology,  metabolism*
Immunohistochemistry
Molecular Sequence Data
Muscle Contraction / drug effects
Oligopeptides / chemistry*
Protein Precursors / chemistry,  genetics
Recombinant Proteins / biosynthesis,  chemistry,  pharmacology
Restriction Mapping
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Grant Support
ID/Acronym/Agency:
K05MH01427/MH/NIMH NIH HHS; MH50235/MH/NIMH NIH HHS; NS31609/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Mytilus inhibitory peptide; 0/Oligopeptides; 0/Protein Precursors; 0/Recombinant Proteins

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