Document Detail

Apicidin, a histone deacetylase inhibitor, induces differentiation of HL-60 cells.
MedLine Citation:
PMID:  12490313     Owner:  NLM     Status:  MEDLINE    
The fungal metabolite apicidin (cyclo(N-O-methyl-L-tryptophanyl-L-isoleucinyl-D-pipecolinyl-L-2-amino-8-oxodecanoyl)) inhibited the growth of HL-60 cells in a concentration-dependent manner (100-1000 nM). At higher concentrations (>300 nM), cell death was induced. At 100 nM, it induced hyperacetylation of histone H4 time-dependently, while trichostatin A induced transient hyperacetylation. Apicidin (10-100 nM) increased the cells having nitroblue tetrazolium-reducing activity and expressing CD11b but not CD14 and CD15. The expression of CD11b by apicidin was long lasting, while that by trichostatin A was transient. In K562 cells, apicidin at 10-100 nM did not inhibit cell growth nor express CD11b, CD14 and CD15. Our findings indicate that apicidin inhibits proliferation and induces the early stage of differentiation of HL-60 cells.
JangJa Hong; Kenji Ishihara; Kouya Yamaki; Kenji Hiraizumi; Tadao Ohno; Jong Woong Ahn; OkPyo Zee; Kazuo Ohuchi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  189     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-12-19     Completed Date:  2003-02-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  197-206     Citation Subset:  IM    
Laboratory of Pathophysiological Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
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MeSH Terms
Acetylation / drug effects
Antigens, CD11b / analysis
Antigens, CD14 / analysis
Antigens, CD15 / analysis
Apoptosis / drug effects
Cell Differentiation / drug effects
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology*
HL-60 Cells / cytology,  drug effects*
Histone Deacetylase Inhibitors*
Histones / metabolism
K562 Cells / drug effects
Neoplasm Proteins / antagonists & inhibitors*,  metabolism
Peptides, Cyclic / pharmacology*
Protein Processing, Post-Translational / drug effects
Reg. No./Substance:
0/Antigens, CD11b; 0/Antigens, CD14; 0/Antigens, CD15; 0/Enzyme Inhibitors; 0/Histone Deacetylase Inhibitors; 0/Histones; 0/Neoplasm Proteins; 0/Peptides, Cyclic; 0/apicidin

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