| Apical and basolateral EGF receptors regulate gastric mucosal paracellular permeability. | |
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MedLine Citation:
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PMID: 11208549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies found that monolayers formed from canine oxyntic epithelial cells in primary culture displayed remarkable resistance to apical acidification and both mitogenic and migratory responses to epidermal growth factor (EGF) treatment. In our present studies, we found that EGF increased transepithelial resistance (TER) but not short-circuit current in these monolayers. Parallel effects of EGF on decreasing mannitol flux and increasing TER implicate direct regulation of paracellular permeability. EGF acting at either apical and basolateral receptors rapidly increased TER, but the apical response was sustained whereas the basolateral response was transient. (125)I-labeled EGF binding revealed specific apical binding, but receptor numbers were 25-fold lower than on the basolateral surface. Both apical and basolateral EGF activated tyrosine phosphorylation of EGF receptors (EGFR), beta-catenin, and cellular substrate as evident on confocal microscopy. Although apical EGF activated a lesser degree of receptor autophosphorylation than basolateral EGF, phosphorylation of beta-catenin was equally prominent with apical and basolateral receptor activation. Together, these findings indicate that functional apical and basolateral EGFR exist on primary canine gastric epithelial cells and that these receptors regulate paracellular permeability. The sustained effect of apical EGFR activation and prominent phosphorylation of beta-catenin suggest that apical EGFR may play a key role in this regulation. |
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Authors:
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M C Chen; J Goliger; N Bunnett; A H Soll |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 280 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-03-14 Completed Date: 2001-04-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G264-72 Citation Subset: IM |
Affiliation:
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CURE: Digestive Diseases Research Center, West Los Angeles Veterans Affairs Medical Center, School of Medicine, University of California, Los Angeles 70073, California. mcychen@ucla.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / pharmacology Cell Membrane / metabolism Cells, Cultured Dogs Electric Impedance Epidermal Growth Factor / metabolism, pharmacology Gap Junctions / metabolism Gastric Mucosa / cytology, drug effects, metabolism*, physiology Intracellular Membranes / metabolism Permeability Phosphorylation Receptor, Epidermal Growth Factor / immunology, physiology* Tyrosine / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK-19984/DK/NIDDK NIH HHS; DK-30444/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 55520-40-6/Tyrosine; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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