Document Detail

Apheresis techniques for collection of peripheral blood progenitor cells.
MedLine Citation:
PMID:  15556469     Owner:  NLM     Status:  MEDLINE    
The combination of effective mobilisation protocols and efficient use of apheresis machines has caused peripheral blood progenitor cells (PBPC) transplantation to grow rapidly. The development of apheresis technology has improved over the years. Today PBSC procedures have changed towards systems to minimise operator interaction and to reduce the collection of undesired cells such as polymorphonuclear cells and platelets using functionally closed, sterile environments for PBSC collection in keeping with Good Manufacturing Practice guidelines. Blood cell separators with continuous flow technique allow the processing of more blood than intermittent flow devices resulting in higher PBSC yields. Large volume leukapheresis with the processing of 3-4-fold donor's/patient's blood volume can increase the number of collected progenitor cells. Therefore, intermittent flow cell separators are indicated if only single vein access is available. Anticoagulant induced hypocalcaemia is an often observed side effect in long lasting PBPC harvesting and monitoring of electrolytes should be performed especially at the end of the apheresis procedure to supplement low levels of potassium, calcium or magnesium. Refinement and improvement of collection techniques continue to add to the armamentarium of current approaches for cancer and non-malignant conditions and will enable future strategies.
Rainer Moog
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis     Volume:  31     ISSN:  1473-0502     ISO Abbreviation:  Transfus. Apher. Sci.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-23     Completed Date:  2005-02-17     Revised Date:  2006-06-07    
Medline Journal Info:
Nlm Unique ID:  101095653     Medline TA:  Transfus Apher Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  207-20     Citation Subset:  T    
Institute for Transfusion Medicine, University Clinics Essen, Hufelandstrasse 55, Essen 45122, Germany.
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MeSH Terms
Blood Component Removal / instrumentation,  methods*
Blood Platelets / cytology
Cell Separation / methods
Hematopoietic Stem Cells / cytology*
Neutrophils / cytology
Stem Cell Transplantation
Tissue and Organ Harvesting / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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