| Apheresis product identification in the transplant center: development of point-of-care protocols for extended blood typing of stem cell apheresis products. | |
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MedLine Citation:
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PMID: 21927033 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Transfusion of the 'wrong' stem cell product would almost inevitably be lethal, yet assays to confirm the contents of the product bag, except by checking labels and paperwork, are lacking. To increase the likelihood that a product mix-up would be detected in the transplant center, we developed a simple protocol for extended blood typing and hence, for confirmation of donor/product identity, on a tube segment. Apheresis samples were applied, directly or after erythrocyte enrichment, to commercially available blood typing assays, including lateral flow cards and gel agglutination cards. Without sample modification, low hematocrit and high leukocyte count obviated definitive blood typing. Using the most simple erythrocyte enrichment protocol, that is, centrifugation, reliable blood group analysis became possible with either assay. Other, more cumbersome pre-analytical protocols were also successful but provided no advantage. The preferred method was validated on 100 samples; ABD was correctly identified in 100% of cases. Of the other Rh Ags, all except two 'small e', in both cases in heterozygous individuals, were detected; there were no false positives. A simple, inexpensive point-of-care assay for extended blood typing of apheresis products is available, which can reduce the fatal risk of administering the wrong stem cell product.Bone Marrow Transplantation advance online publication, 19 September 2011; doi:10.1038/bmt.2011.182. |
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Authors:
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C Cummerow; P Schwind; M Spicher; G Spohn; C Geisen; E Seifried; H Bönig |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-19 |
Journal Detail:
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Title: Bone marrow transplantation Volume: - ISSN: 1476-5365 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8702459 Medline TA: Bone Marrow Transplant Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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German Red Cross Blood Center and Institute for Transfusion Medicine and Immunohematology of the Goethe-University, Frankfurt, Germany. |
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