|Apelin is decreased with human preterm and term labor and regulates prolabor mediators in human primary amnion cells.|
|PMID: 23314958 Owner: NLM Status: MEDLINE|
|A critical role of proinflammatory mediators including cytokines, prostaglandins, and extracellular matrix remodeling enzymes in the processes of human labor and delivery, at term and preterm, has been demonstrated. In nongestational tissues, apelin plays an important role in a number of physiologic processes, including the regulation of inflammation. However, the role and regulation of apelin and the apelin receptor (APJ) in human gestational tissues are not known. The aims of this study were to determine the effect of (i) preterm and term labor on apelin and APJ expression in human gestational tissues and (ii) apelin small interfering RNA (siRNA) knockdown in human primary amnion cells on prolabor mediators. Human placenta and fetal membranes were collected from term nonlaboring women and women after spontaneous labor and delivery. Preterm and term spontaneous labor were associated with significantly lower apelin expression in fetal membranes. On the other hand, there was no effect of labor on APJ expression and no effect of term labor on placental apelin or APJ expression. Transfection of primary amnion cells with apelin siRNA was associated with significantly increased interleukin (IL)-1β-induced IL-6 and IL-8 release and cyclooxygenase-2 messenger RNA (mRNA) expression and resultant prostaglandin E2 and prostaglandin F2α release. There was no effect of apelin siRNA on matrix metalloproteinase (MMP)-9 mRNA expression and pro MMP-9 release. In summary, human labor downregulates apelin expression in human fetal membranes. Furthermore, a role of apelin in the regulation of proinflammatory and prolabor mediators in human fetal membranes is supported by our studies.|
|Ratana Lim; Gillian Barker; Clyde Riley; Martha Lappas|
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|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2013-01-11|
|Title: Reproductive sciences (Thousand Oaks, Calif.) Volume: 20 ISSN: 1933-7205 ISO Abbreviation: Reprod Sci Publication Date: 2013 Aug|
|Created Date: 2013-07-17 Completed Date: 2014-01-09 Revised Date: 2014-08-05|
Medline Journal Info:
|Nlm Unique ID: 101291249 Medline TA: Reprod Sci Country: United States|
|Languages: eng Pagination: 957-67 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Cyclooxygenase 2 / genetics, metabolism
Dinoprost / metabolism
Dinoprostone / metabolism
Inflammation Mediators / metabolism
Intercellular Signaling Peptides and Proteins / genetics, metabolism*
Interleukin-1beta / genetics, metabolism
Interleukin-6 / genetics, metabolism
Interleukin-8 / genetics, metabolism
Matrix Metalloproteinase 9 / genetics, metabolism
Placenta / metabolism
Premature Birth / genetics, metabolism*
RNA, Messenger / metabolism
Receptors, G-Protein-Coupled / metabolism
Term Birth / genetics, metabolism*
|0/APLN protein, human; 0/APLNR protein, human; 0/IL6 protein, human; 0/IL8 protein, human; 0/Inflammation Mediators; 0/Intercellular Signaling Peptides and Proteins; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; B7IN85G1HY/Dinoprost; EC 220.127.116.11/Cyclooxygenase 2; EC 18.104.22.168/PTGS2 protein, human; EC 22.214.171.124/MMP9 protein, human; EC 126.96.36.199/Matrix Metalloproteinase 9; K7Q1JQR04M/Dinoprostone|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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