Document Detail


Apelin-13 limits infarct size and improves cardiac post-ischemic mechanical recovery only if given after ischemia.
MedLine Citation:
PMID:  21378145     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We studied whether apelin-13 is cardioprotective against ischemia/reperfusion injury if given as either a pre- or postconditioning mimetic, and whether the improved post-ischemic mechanical recovery induced by apelin-13 depends only on the reduced infarct size or also on a recovery of function of the viable myocardium. We also studied whether nitric oxide (NO) is involved in apelin-induced protection and whether the reported ischemia-induced overexpression of the apelin receptor APJ plays a role in cardioprotection. Langendorff perfused rat hearts underwent 30-min of global ischemia and 120 min of reperfusion. Left ventricular pressure was recorded. Infarct size and LDH release were determined to evaluate the severity of myocardial injury. Apelin-13 was infused at 0.5 μM concentration for 20 min either before ischemia or in early reperfusion, without and with nitric oxide-synthase inhibition by L-N(G)-Nitroarginine (L-NNA). In additional experiments, before ischemia also 1 μM apelin-13 was tested. APJ protein level was measured before and after ischemia. While before ischemia apelin-13 (0.5 and 1 μM) was ineffective, after ischemia it reduced infarct size from 54±2% to 26±4% of risk area (p<0.001) and limited the post-ischemic myocardial contracture (p<0.001). L-NNA alone increased post-ischemic myocardial contracture. This increase was attenuated by apelin-13, which however, was unable to reduce infarct size. Ischemia increased APJ protein level after 15-min reperfusion, i.e. after most of reperfusion injury has occurred. Apelin-13 protects the heart only if given after ischemia. In this protection NO plays an important role. Apelin-13 efficiency as postconditioning mimetic cannot be explained by the increased APJ level.
Authors:
Raffaella Rastaldo; Sandra Cappello; Anna Folino; Giovanni N Berta; Andrea E Sprio; Gianni Alberto Losano; Michele Samaja; Pasquale Pagliaro
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-4
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  -     ISSN:  1522-1539     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1UNIVERSITY OF TURIN.
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