Document Detail

Apatite induced renal epithelial injury: insight into the pathogenesis of kidney stones.
MedLine Citation:
PMID:  18499159     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Kidney stone formation is associated with the deposition of hydroxyapatite as subepithelial plaques or tubular deposits in the renal papillae. We investigated the effect of renal epithelial exposure to hydroxyapatite crystals in vitro to develop an insight into the pathogenesis of kidney stones.
MATERIALS AND METHODS: NRK52E cells (No. CRL-1571, ATCC) were exposed to 67 or 133 microg/cm(2) hydroxyapatite (No. 21223, Sigma-Aldrich) or calcium oxalate monohydrate crystals (No. 27609, BDH Industries, Poole, United Kingdom). In some studies cells were also exposed to crystals from the basal side. After 3 or 6 hours of exposure medium was analyzed for lactate dehydrogenase, 8-isoprostane and H(2)O(2). Medium collected after cell exposure on the apical side was also analyzed for the production of monocyte chemoattractant protein-1 and prostaglandin E2. Cells were stained with DAPI to determine apoptotic activity and examined by scanning electron microscopy to observe crystal-cell interaction.
RESULTS: Cell exposure to hydroxyapatite resulted in H(2)O(2) and 8-isoprostane production as well as in lactate dehydrogenase release. Apical exposure appeared more provocative and injurious than basal exposure. Exposure to hydroxyapatite for 6 hours resulted in increased apoptotic activity. Apical exposure also resulted in increased monocyte chemoattractant protein-1 and prostaglandin E2 production.
CONCLUSIONS: Cell exposure to hydroxyapatite crystals induced oxidative stress and lipid peroxidation. It caused up-regulation of the inflammation mediators that may be responsible for the kidney inflammation in patients with stones that is associated with tubular hydroxyapatite deposition. It may also have a role in the eruption of subepithelial Randall's plaques to the papillary surface.
Carla Escobar; Karen J Byer; Hassan Khaskheli; Saeed R Khan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-05-21
Journal Detail:
Title:  The Journal of urology     Volume:  180     ISSN:  1527-3792     ISO Abbreviation:  J. Urol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-12     Completed Date:  2008-08-11     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  379-87     Citation Subset:  AIM; IM    
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MeSH Terms
Cells, Cultured
Hydroxyapatites* / metabolism
Kidney / metabolism
Kidney Calculi / etiology*,  metabolism
Reactive Oxygen Species
Urothelium / metabolism
Grant Support
Reg. No./Substance:
0/Hydroxyapatites; 0/Reactive Oxygen Species

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