| Aortic smooth muscle cells migration and the role of metalloproteinases and hyaluronan. | |
| | |
MedLine Citation:
|
PMID: 18661340 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human aortic smooth muscle cells (AoSMCs) change their extracellular matrix composition during aging, with direct effects on cellular events and cell migration. For example, active matrix metalloproteinase-2 (MMP-2) is synthesized only by young AoSMCs, whereas aged cells produce only the inactive zymogen form. The pro-MMP-2 activation in young cells depends on an increase in membrane type 1 matrix metalloproteinase content. Furthermore, transcripts coding for tissue inhibitor of metalloproteinases (TIMPs) were upregulated in aged cells, and the increase of TIMPs also could prevent pro-MMP-2 activation. As consequence of these situations, young AoSMCs possess a higher migratory capability than aged cells on gelatin support. These data are confirmed by adding TIMP-1 and TIMP-2 to young cells which reproduces aged AoSMCs migratory behavior. The opposite effect was obtained in young cells silencing MMP-2 and TIMP-2. |
| | |
Authors:
|
Davide Vigetti; Paola Moretto; Manuela Viola; Anna Genasetti; Manuela Rizzi; Evgenia Karousou; Moira Clerici; Barbara Bartolini; Francesco Pallotti; Giancarlo De Luca; Alberto Passi |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Connective tissue research Volume: 49 ISSN: 1607-8438 ISO Abbreviation: Connect. Tissue Res. Publication Date: 2008 |
Date Detail:
|
Created Date: 2008-07-28 Completed Date: 2008-09-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: United States |
Other Details:
|
Languages: eng Pagination: 189-92 Citation Subset: IM |
Affiliation:
|
Department of Experimental Biomedical and Clinical Sciences, University of Insubria, Varese, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD44 / metabolism* Aorta Cell Movement Humans Hyaluronic Acid / metabolism* Matrix Metalloproteinase 2 / metabolism* Muscle, Smooth, Vascular / cytology*, physiology Myocytes, Smooth Muscle / enzymology, physiology* Tissue Inhibitor of Metalloproteinases / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD44; 0/Tissue Inhibitor of Metalloproteinases; 9004-61-9/Hyaluronic Acid; EC 3.4.24.24/Matrix Metalloproteinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dysregulation of matrix metalloproteinases and their tissue inhibitors by S100A4.
Next Document: Role of cysteine cathepsins in matrix degradation and cell signalling.