Document Detail


Aortic smooth muscle cells contain vimentin instead of desmin.
MedLine Citation:
PMID:  7019916     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The 10-nm filament subunit proteins of smooth muscle cells in the mammalian aorta were analyzed by immunofluorescence and by two-dimensional gel electrophoresis. Indirect immunofluorescence studies on frozen sections of aorta showed intense staining of the tunica media by antibodies to vimentin, whereas no reaction could be detected with antibodies to desmin. Crude preparations from the tunica media contain a protein with molecular weight and isoelectric point similar to those of vimentin. Desmin was not detected on these same two-dimensional gels. We conclude that aortic smooth muscle cells contain vimentin as the predominant form of 10-nm filament protein subunit, unlike other muscle cells in which desmin predominates. Other immunofluorescence results indicate that the aorta may differ from smaller blood vessels which appear to contain both desmin and vimentin.
Authors:
E D Frank; L Warren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  78     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1981 May 
Date Detail:
Created Date:  1981-09-25     Completed Date:  1981-09-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3020-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Aorta / analysis*
Cell Line
Cricetinae
Desmin
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Humans
Immunoassay
Kidney
Molecular Weight
Muscle Proteins / analysis*
Muscle, Smooth, Vascular / analysis*
Vimentin
Grant Support
ID/Acronym/Agency:
CA-19130/CA/NCI NIH HHS; CA-27458/CA/NCI NIH HHS; HL-14217/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Desmin; 0/Muscle Proteins; 0/Vimentin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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