Document Detail

Anxa2 plays a critical role in enhanced invasiveness of the multidrug resistant human breast cancer cells.
MedLine Citation:
PMID:  19764771     Owner:  NLM     Status:  MEDLINE    
Multidrug resistance (MDR) is the major cause of failure in cancer chemotherapy. Recent reports even suggest that MDR is associated with elevated invasion and metastasis of tumor cells. In the current study, we used a proteomic approach to identify genes that play an important role in MDR induced cell migration. 2D-PAGE and MALDI-TOF/MS-based proteomics approach were used to separate and identify differentially expressed proteins between MCF-7 and MCF-7/ADR, a p-glycoprotein-overexpressing adriamycin-resistance breast cancer cell line. Annexin a2 (Anxa2) was identified as highly expressed in MCF-7/ADR cells, but not in MCF-7 cells. Small interference RNA-mediated gene suppression demonstrated that Anxa2 was required for enhanced cell proliferation and invasion of the MCF-7/ADR cells. Down-regulation of Anxa2 alone was not sufficient to revert the cell sensitivity to adriamycin, suggesting that Anxa2 was not required for MDR phenotype. Taken together, our results showed that expression of Anxa2 is enhanced when cancer cells, MCF-7, acquired drug resistance and it plays an essential role in MDR-induced tumor invasion.
Fei Zhang; Lin Zhang; Bin Zhang; Xiyin Wei; Yi Yang; Robert Z Qi; Guoguang Ying; Ning Zhang; Ruifang Niu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of proteome research     Volume:  8     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-06     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5041-7     Citation Subset:  IM    
Key Laboratory of Breast Cancer Prevention and Treatment, Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, PR China.
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MeSH Terms
Annexin A2 / genetics,  metabolism*
Breast Neoplasms* / pathology,  physiopathology
Cell Line, Tumor
Cell Proliferation
Drug Resistance, Multiple / physiology*
Drug Resistance, Neoplasm / physiology*
Electrophoresis, Gel, Two-Dimensional / methods
Image Processing, Computer-Assisted
Molecular Sequence Data
Neoplasm Invasiveness / physiopathology*
RNA, Small Interfering / genetics,  metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Reg. No./Substance:
0/ANXA2 protein, human; 0/Annexin A2; 0/RNA, Small Interfering

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