| Antiviral effect of epigallocatechin gallate on enterovirus 71. | |
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MedLine Citation:
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PMID: 19537794 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidative stress is known to be a determinant of a host's susceptibility to pathogens. Natural compounds with antioxidant activity may provide a preventive measure against infection. Tea polyphenols were evaluated for their ability to inhibit enterovirus 71 (EV71) replication in Vero cell culture. Among the polyphenolic compounds tested, epigallocatechin gallate (EGCG) and gallocatechin gallate (GCG) potently inhibited replication of EV71. EGCG and GCG reduced the titer of infectious progeny virus by 95%. Quantitative RT-PCR analysis also revealed that EGCG suppressed replication of genomic RNA. It was accompanied by an increased cytoprotective effect. EGCG and GCG caused 5-fold increase in the viability of EV71-infected cells. The viral inhibitory effect correlated well with the antioxidant capacity of polyphenol. Mechanistically, EV71 infection led to increased oxidative stress, as shown by increased dichlorofluorescein and MitoSOX Red fluorescence. Upon EGCG treatment, reactive oxygen species (ROS) generation was significantly reduced. Consistent with this, EV71 replication was enhanced in glucose-6-phosphate dehydrogenase deficient cells, and such enhancement was largely reversed by EGCG. These findings suggest that EGCG may suppress viral replication via modulation of cellular redox milieu. |
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Authors:
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Hung-Yao Ho; Mei-Ling Cheng; Shiue-Fen Weng; Yann-Lii Leu; Daniel Tsun-Yee Chiu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 57 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-15 Completed Date: 2009-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6140-7 Citation Subset: IM |
Affiliation:
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Graduate Institute of Medical Biotechnology and Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Kwei-san, Taoyuan, Taiwan. hoh01@mail.cgu.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / pharmacology* Antiviral Agents / pharmacology* Catechin / analogs & derivatives*, pharmacology Cell Line Cercopithecus aethiops Enterovirus A, Human / drug effects*, genetics, physiology Fibroblasts / virology Glycogen Storage Disease Type I Humans Male Oxidative Stress / drug effects RNA, Viral / biosynthesis Reactive Oxygen Species / antagonists & inhibitors Reverse Transcriptase Polymerase Chain Reaction Vero Cells Virus Replication / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Antiviral Agents; 0/RNA, Viral; 0/Reactive Oxygen Species; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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