| Antiviral, anticellular and enzyme-inducing activities of interferons in RD-114 cells. | |
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MedLine Citation:
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PMID: 6194249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RD-114 is a human sarcoma-derived cell line which is chronically infected with the RD-114 retrovirus. In a previous study, we found that treatment of these cells with human interferon-alpha or human interferon-gamma causes a marked inhibition of RD-114 virus production, but that the replication of exogenous vesicular stomatitis or encephalomyocarditis virus is not impaired. In the present study, we report that neither type of interferon has strong inhibitory effects on DNA synthesis or on multiplication of the cells. We also failed to detect a double-stranded RNA-dependent protein kinase activity in extracts of both interferon-treated and untreated cells. However, a low level of 2',5'-oligoadenylate [2,5(A)] synthetase activity was detectable in extracts of interferon-treated cells. 2,5(A)-dependent endonuclease L activity was detectable in extracts of both untreated and interferon-treated cells. This was probably responsible for the inhibition of protein synthesis observed upon introduction of 2,5(A) to RD-114 cells. In many cells, interferon has been found to induce synthesis of several proteins demonstrable by autoradiographic analysis of slab gels on which extracts of interferon-treated and radiolabelled cells are separated. Using a similar method, no such induced protein synthesis was detectable in interferon-treated RD-114 cells. Our results indicate that RD-114 cells are resistant to most known actions of interferons except for the antiretroviral action to which they are as sensitive as any other cell line. |
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Authors:
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G C Sen; R E Herz; B Y Rubin |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of general virology Volume: 64 (Pt 10) ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 1983 Oct |
Date Detail:
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Created Date: 1983-11-23 Completed Date: 1983-11-23 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2213-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2',5'-Oligoadenylate Synthetase
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biosynthesis Cell Division / drug effects Cell Line Chronic Disease DNA / antagonists & inhibitors Endoribonucleases / biosynthesis Enzyme Induction / drug effects Humans Interferons / pharmacology* Protein Kinases / biosynthesis RNA, Double-Stranded / biosynthesis Retroviridae Infections / enzymology, microbiology* Sarcoma / enzymology, microbiology* Virus Cultivation eIF-2 Kinase |
| Grant Support | |
ID/Acronym/Agency:
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AI-17920/AI/NIAID NIH HHS; CA-08748/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Double-Stranded; 9007-49-2/DNA; 9008-11-1/Interferons; EC 2.7.-/Protein Kinases; EC 2.7.11.1/eIF-2 Kinase; EC 2.7.7.-/2',5'-Oligoadenylate Synthetase; EC 3.1.-/2'-5'-oligo(A)-dependent endonuclease; EC 3.1.-/Endoribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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