Document Detail

Antiviral action of nitric oxide on dengue virus type 2 replication.
MedLine Citation:
PMID:  16963759     Owner:  NLM     Status:  MEDLINE    
Recently, nitric oxide (NO) has been shown to suppress dengue virus (DENV) RNA and protein accumulation in infected cells. In this report, the potential target of the inhibitory effect of NO was studied at the molecular level. The NO donor, S-nitroso-N-acetylpenicillamine (SNAP), showed an inhibitory effect on RNA accumulation at around 8-14 h post-infection, which corresponded to the step of viral RNA synthesis in the DENV life cycle. The activity of the viral replicase isolated from SNAP-treated DENV-2-infected cells was suppressed significantly compared with that of the negative-control N-acetyl-DL-penicillamine (NAP)-treated cells. Further investigations on the molecular target of NO action showed that the activity of recombinant DENV-2 NS5 in negative-strand RNA synthesis was affected in the presence of 5 mM SNAP in in vitro RNA-dependent RNA polymerase (RdRp) assays, whereas the RNA helicase activity of DENV-2 NS3 was not inhibited up to a concentration of 15 mM SNAP. These results suggest that the inhibitory effect of NO on DENV infection is partly via inhibition of the RdRp activity, which then downregulates viral RNA synthesis.
Ratree Takhampunya; R Padmanabhan; Sukathida Ubol
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general virology     Volume:  87     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-11     Completed Date:  2006-10-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3003-11     Citation Subset:  IM    
Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama VI Road, Bangkok 10400, Thailand.
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MeSH Terms
Antiviral Agents / pharmacology*
Cell Line
Dengue Virus / drug effects*,  physiology*
Nitric Oxide / pharmacology*
Protein Binding
RNA Helicases / metabolism
RNA, Viral / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Viral Nonstructural Proteins / metabolism
Virus Replication / drug effects*
Grant Support
Reg. No./Substance:
0/Antiviral Agents; 0/RNA, Viral; 0/Viral Nonstructural Proteins; 10102-43-9/Nitric Oxide; EC 2.7.7.-/RNA Helicases

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