Document Detail

Antiviral stilbene 1,2-diamines prevent initiation of hepatitis C virus RNA replication at the outset of infection.
MedLine Citation:
PMID:  21430055     Owner:  NLM     Status:  MEDLINE    
The recent development of a cell culture model of hepatitis C virus (HCV) infection based on the JFH-1 molecular clone has enabled discovery of new antiviral agents. Using a cell-based colorimetric screening assay to interrogate a 1,200-compound chemical library for anti-HCV activity, we identified a family of 1,2-diamines derived from trans-stilbene oxide that prevent HCV infection at nontoxic, low micromolar concentrations in cell culture. Structure-activity relationship analysis of ~ 300 derivatives synthesized using click chemistry yielded compounds with greatly enhanced low nanomolar potency and a > 1,000:1 therapeutic ratio. Using surrogate models of HCV infection, we showed that the compounds selectively block the initiation of replication of incoming HCV RNA but have no impact on viral entry, primary translation, or ongoing HCV RNA replication, nor do they suppress persistent HCV infection. Selection of an escape variant revealed that NS5A is directly or indirectly targeted by this compound. In summary, we have identified a family of HCV inhibitors that target a critical step in the establishment of HCV infection in which NS5A translated de novo from an incoming genomic HCV RNA template is required to initiate the replication of this important human pathogen.
Pablo Gastaminza; Suresh M Pitram; Marlene Dreux; Larissa B Krasnova; Christina Whitten-Bauer; Jiajia Dong; Josan Chung; Valery V Fokin; K Barry Sharpless; Francis V Chisari
Related Documents :
21114585 - Hbsag-negative mono-infection with hepatitis b virus genotype g.
19876475 - Profile of clients tested hiv positive in a voluntary counseling and testing center of ...
21375685 - Different distributions of hepatitis c virus genotypes among hiv-infected patients with...
21258665 - The impact of sirolimus on hepatitis c recurrence after liver transplantation.
22846195 - Early versus delayed initiation of antiretroviral therapy for indian hiv-infected indiv...
21177735 - Mother to child transmission of hiv among zimbabwean women who seroconverted postnatall...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-03-23
Journal Detail:
Title:  Journal of virology     Volume:  85     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-09     Completed Date:  2011-07-13     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5513-23     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antiviral Agents / chemistry,  isolation & purification,  pharmacology*,  toxicity
Cell Line
Cell Survival / drug effects
Diamines / chemistry,  isolation & purification,  pharmacology*,  toxicity
Drug Evaluation, Preclinical / methods
Drug Resistance, Viral
Hepacivirus / drug effects*
Hepatocytes / drug effects,  virology
Microbial Sensitivity Tests
RNA, Viral / metabolism
Stilbenes / chemistry,  isolation & purification,  pharmacology*,  toxicity
Structure-Activity Relationship
Viral Nonstructural Proteins / genetics,  metabolism
Virus Replication / drug effects*
Grant Support
Reg. No./Substance:
0/Antiviral Agents; 0/Diamines; 0/NS-5 protein, hepatitis C virus; 0/RNA, Viral; 0/Stilbenes; 0/Viral Nonstructural Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Activation of the alphavirus spike protein is suppressed by bound E3.
Next Document:  Sequential immunization with a subtype B HIV-1 envelope quasispecies partially mimics the in vivo de...