Document Detail


Antiviral Specificity of the Solea senegalensis Mx Protein Constitutively Expressed in CHSE-214 Cells.
MedLine Citation:
PMID:  22886190     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Interferons play a key role in fish resistance to viral infections by inducing the expression of antiviral proteins, such as Mx. The aim of the present study was to test the antiviral activity of the Senegalese sole Mx protein (SsMx) against RNA and DNA viruses pathogenic to fish, i.e. the infectious pancreatic necrosis virus (IPNV, dsRNA), the viral haemorrhagic septicaemia virus (VHSV, ssRNA), and the European sheatfish virus (ESV, dsDNA), using a CHSE-214 cell clone expressing this antiviral protein. A strong inhibition of IPNV and VHSV replication was recorded in SsMx-expressing cells, as has been shown by the virus yield reduction and the decrease in the synthesis of the viral RNA encoding the polyprotein (for IPNV) and the nucleoprotein (for VHSV). The titres of these viruses replicating on SsMx-expressing cells were 100 times lower than those recorded on non-transfected cells. In contrast, SsMx did not inhibit ESV replication since no significant differences were observed regarding the virus yield or the major capsid protein gene transcription in transfected and non-transfected cells.
Authors:
Daniel Alvarez-Torres; Esther Garcia-Rosado; M Alejandra Fernandez-Trujillo; Julia Bejar; M Carmen Alvarez; Juan J Borrego; M Carmen Alonso
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-11
Journal Detail:
Title:  Marine biotechnology (New York, N.Y.)     Volume:  -     ISSN:  1436-2236     ISO Abbreviation:  Mar. Biotechnol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892712     Medline TA:  Mar Biotechnol (NY)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Microbiology, Faculty of Sciences, University of Malaga, Campus Teatinos, 29071, Malaga, Spain.
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