| Antitumour activity of Salmonella typhimurium VNP20047 in B16(F10) murine melanoma model. | |
| | |
MedLine Citation:
|
PMID: 15448746 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A tumour therapy is proposed based on attenuated Salmonella typhimurium VNP20047 expressing the Escherichia coli cytosine deaminase gene. VNP20047 was administered intravenously to B16(F10) melanoma-bearing C57BL/6 mice. VNP20047 proliferated within tumours and livers regardless of the initial inoculum dose. After 10 days the number of bacteria increased in livers up to 4.2 x 10(6) cfu/g and decreased in tumours down to 5.9 x 10(6) cfu/g. VNP20047 at 1 x 10(5) cfu/mouse, when combined with 5-fluorocytosine, inhibited tumour growth by 85% without prolonging animal survival. Histology studies revealed severe lesions in tumours and livers. These data suggest that S. typhimurium VNP20047 induced inflammatory responses, even though the strain was attenuated. |
| | |
Authors:
|
Joanna Jazowiecka-Rakus; Stanisław Szala |
Related Documents
:
|
19561956 - Simultaneous acute appendicitis and ectopic pregnancy. 21781266 - Antithrombotic treatment for recurrent pregnancy loss? 6955966 - Acute leukaemia in pregnancy. a case report and discussion on modern management. 9569356 - Endoscopic sphincterotomy for the treatment of gallstone pancreatitis during pregnancy. 8115596 - Genetic conflicts in human pregnancy. 12386356 - The potential of preoperative beta-hcg and progesterone levels to predict failure of la... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Acta biochimica Polonica Volume: 51 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 2004 |
Date Detail:
|
Created Date: 2004-09-27 Completed Date: 2005-03-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: Poland |
Other Details:
|
Languages: eng Pagination: 851-6 Citation Subset: IM |
Affiliation:
|
Department of Molecular Biology, Centre of Oncology, Maria Skłodowska-CurieMemorial Institute, Gliwice, Poland. jjazowiecka@io.gliwice.pl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antimetabolites / therapeutic use Colony Count, Microbial Cytosine Deaminase / genetics Escherichia coli / enzymology, genetics Flucytosine / therapeutic use Gene Therapy / adverse effects, methods* Genes, Bacterial Inflammation / etiology, pathology Liver / microbiology, pathology Melanoma, Experimental / microbiology, pathology, therapy* Mice Mice, Inbred C57BL Salmonella typhimurium / genetics*, growth & development, isolation & purification, pathogenicity Virulence / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Antimetabolites; 2022-85-7/Flucytosine; EC 3.5.4.1/Cytosine Deaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influence of BCR/ABL fusion proteins on the course of Ph leukemias.
Next Document: Role of rpoS in the regulation of glyoxalase III in Escherichia coli.