| Antitumor properties of aloe-emodin and induction of transglutaminase 2 activity in B16-F10 melanoma cells. | |
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MedLine Citation:
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PMID: 20624404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: Aloe-emodin (AE), a natural hydroxyanthraquinone compound, has been reported as a potential anticancer agent. We studied the antineoplastic properties of AE on highly metastatic B16-F10 melanoma murine cells. MAIN METHODS: Cell proliferation was assessed by cell counting and viability was investigated using MTT and Trypan Bleu exclusion tests. As a growth marker, we determined intracellular polyamine levels by high performance liquid chromatography. Then, we evaluated transglutaminase 2 (TG2) activity, protoporphyrin IX accumulation and melanin content as differentiative markers. Tyrosinase activity was checked by DOPA-staining assay. The antimetastatic effect of AE was evaluated by means of a series of in vitro metastatic assays, including aggregation, wound healing migration, adhesion, 3D-invasion, circular invasion and the Boyden chamber invasion assays. Gelatin zymography was performed to evaluate metalloproteinase activities. KEY FINDINGS: Our results demonstrated inhibitory effects of AE on melanoma cell proliferation and invasion power, accompanied by the stimulation of cell differentiation parameters. Cell differentiation correlated with a remarkable increase of the activity of the transamidating form of TG2, with a significative enhancement of cell adhesion and aggregation. Impaired invasion was paralleled by the decrease of the secretion of matrix metalloproteinase-9. SIGNIFICANCE: The overall data confirm a remarkable antiproliferative, antimetastatic and differentiative capability of this anthraquinone. Results suggest that AE appears particularly promising for its potential application in the newborn differentiation therapy of cancer. |
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Authors:
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Claudio Tabolacci; Alessandro Lentini; Palma Mattioli; Bruno Provenzano; Serafina Oliverio; Fabrizio Carlomosti; Simone Beninati |
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Publication Detail:
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Type: Journal Article Date: 2010-07-16 |
Journal Detail:
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Title: Life sciences Volume: 87 ISSN: 1879-0631 ISO Abbreviation: Life Sci. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-20 Completed Date: 2010-09-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 316-24 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Biology, University Tor Vergata, Rome, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anthraquinones / pharmacology* Antineoplastic Agents, Phytogenic / pharmacology* Cell Adhesion / drug effects Cell Aggregation / drug effects Cell Line, Tumor Cell Proliferation / drug effects* Cell Survival / drug effects Enzyme Induction GTP-Binding Proteins / biosynthesis*, metabolism Intramolecular Oxidoreductases / metabolism Matrix Metalloproteinases / metabolism Melanins / biosynthesis Melanoma, Experimental / enzymology, pathology Mice Protoporphyrins / metabolism Transglutaminases / biosynthesis*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anthraquinones; 0/Antineoplastic Agents, Phytogenic; 0/Melanins; 0/Protoporphyrins; 0/aloe emodin; 553-12-8/protoporphyrin IX; EC 2.3.2.-/transglutaminase 2; EC 2.3.2.13/Transglutaminases; EC 3.4.24.-/Matrix Metalloproteinases; EC 3.6.1.-/GTP-Binding Proteins; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.3.12/dopachrome isomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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