Document Detail


Antitumor effects of a selenium heteropoly complex in K562 cells.
MedLine Citation:
PMID:  19443941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to investigate the anti-tumor effects and mechanism of the selenium heteropoly compound (C(2)H(10)N(2))(5)(NH(4))(4)H(2)[Se(2)W(10)V(8)O(62)].9H(2)O (SeWV) in K562 cells. The results showed that 0.32-10.15 x 10(-3) mmol/l SeWV could significantly inhibit the proliferation of K562 cells in vitro, as determined by the MTT assay, with IC(50) values of 3.07 and 2.69 x 10(-3) mmol/l after 48 and 72 h of treatment with SeWV, respectively. Studies of the cell cycle indicated that SeWV could induce K562 cells gathered in the G(2)/M phase upon treatment for 24 and 48 h, and a significant sub-G1 peak was evident at 0.32 and 2.54 x 10(-3) mmol/l after treatment for 24 h. Morphological observations revealed typical apoptotic features. SeWV caused the accumulation of Ca(2+), Mg(2+) and ROS, and the reduction of pH and mitochondrial membrane potential (MMP) in K562 cells as evidenced by confocal laser scanning microscopy. Experiments also showed that the expression of Bcl-2 was significantly inhibited, but Bax was increased by SeWVat 5.07 x 10(-3) mmol/l. Additionally, the content of cytochrome-C was increased after treatment for 24 h. The experiment implied that SeWV had anti-tumor activity and that its mechanism was partially attributable to the induction of cell cycle distribution and apoptosis that was induced by a change in intracellular ion homeostasis.
Authors:
Yang Jun-Ying; Xu Cun-Shuan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pharmacological reports : PR     Volume:  61     ISSN:  1734-1140     ISO Abbreviation:  Pharmacol Rep     Publication Date:    2009 Mar-Apr
Date Detail:
Created Date:  2009-05-15     Completed Date:  2009-07-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234999     Medline TA:  Pharmacol Rep     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  288-95     Citation Subset:  IM    
Affiliation:
Key Laboratory for Cell Differentiation Regulation, College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, PR China. Junyingyang@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Calcium / metabolism
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cytochromes c / analysis
Humans
K562 Cells
Magnesium / metabolism
Proto-Oncogene Proteins c-bcl-2 / analysis
Selenium Compounds / pharmacology*
Chemical
Reg. No./Substance:
0/(C(2)H(10)N(2))(5)(NH(4))(4)H(2)(Se(2)W(10)V(8)O(62)).9H(2)O; 0/Antineoplastic Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/Selenium Compounds; 7439-95-4/Magnesium; 7440-70-2/Calcium; 9007-43-6/Cytochromes c

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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