| Antitumor effects of polysorbate-80 coated gemcitabine polybutylcyanoacrylate nanoparticles in vitro and its pharmacodynamics in vivo on C6 glioma cells of a brain tumor model. | |
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MedLine Citation:
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PMID: 19401168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polybutylcyanoacrylate (PBCA) nanoparticles coated with polysorbate-80 have been extensively studied for delivery of drugs into the animal models; however, 1% polysorbate-80 coated gemcitabine PBCA nanoparticles (GCTB-PBCA-NPs) remain unknown. In this study, we investigated the inhibitory effects of brain targeted 1% polysorbate-80 coated GCTB-PBCA-NPs on C6 glioma cells in vitro and in vivo. GCTB-PBCA-NPs were prepared by emulsion polymerization and freeze drying. C6 glioma cells treated with 1% polysorbate-80 coated GCTB-PBCA-NPs showed poor growth with less cell density and increased detachment. Cell morphology was also greatly altered with nuclear vacuoles, ruptured cells and dead cells. Meanwhile, by flow cytometry, the numbers of cells treated with 1% polysorbate-80 coated GCTB-PBCA-NPs showed increase in G0/G1 phase and decreased in the S phase (P<0.01) compared with the blank control. CCK-8 assay also showed that GCTB could significantly inhibited cell proliferation in a concentration dependent manner. Finally, various preparations were injected (90 mg preparation per kg body weight) into the brain tumor model, which was produced after inoculating C6 glioma cells into Sprague Dawley (SD) rats for 14 days, it was shown that 1% polysorbate-80 coated GCTB-PBCA-NPs could significantly extend the survival time compared with the saline control (P<0.05). Taken together, 1% polysorbate-80 coated GCTB-PBCA-NPs can effectively inhibit the growth of C6 glioma cells in vitro and enhance antitumor activity on brain tumor in vivo. |
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Authors:
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Chun-Xia Wang; Le-Song Huang; Lian-Bing Hou; Liang Jiang; Zhi-Ting Yan; Yu-Lun Wang; Zhi-Liang Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-20 |
Journal Detail:
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Title: Brain research Volume: 1261 ISSN: 1872-6240 ISO Abbreviation: Brain Res. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-10-30 Completed Date: 2010-01-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 91-9 Citation Subset: IM |
Affiliation:
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Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou, PR China. wcxia@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Brain / drug effects, pathology Brain Neoplasms / drug therapy*, mortality, pathology Cell Cycle / drug effects* Cell Line, Tumor Cell Proliferation / drug effects Cells, Cultured Deoxycytidine / administration & dosage, analogs & derivatives*, therapeutic use Flow Cytometry Glioma / drug therapy*, mortality, pathology Male Nanoparticles / administration & dosage*, therapeutic use Neoplasm Transplantation Polysorbates / administration & dosage*, therapeutic use Rats Rats, Sprague-Dawley Staining and Labeling Survival Rate |
| Chemical | |
Reg. No./Substance:
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0/Polysorbates; 103882-84-4/gemcitabine; 951-77-9/Deoxycytidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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