Document Detail


Antitumor effects of curcin from seeds of Jatropha curcas.
MedLine Citation:
PMID:  12617773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the antitumor effects of curcin from Jatropha curcas. METHODS: Antitumor activity of curcin was tested by MTT assay. The N-glycosidase activity of curcin was determined by characterization of R-fragment in gel. A cell-free system, rabbit reticulocyte lysate, was introduced to quantify the inhibitory activity of curcin on protein biosynthesis. RESULTS: The curcin had a powerful inhibitory action upon protein synthesis in reticulocyte lysate with an IC50 (95 % confidence limits) value of 0.19 (0.11-0.27) nmol/L. The IC50 (95 % confidence limits) of curcin on SGC-7901, Sp2/0, and human hepatoma was 0.23 (0.15-0.32) mg/L, 0.66 (0.35-0.97) mg/L, 3.16 (2.74-3.58) mg/L, respectively. Curcin was found to have no toxic to Hela cells and normal cells (MRC). After the rRNA of ribosome was treated with curcin and aniline at acidic condition, a cleaved R-fragment of approximately 450 nt appeared, but this fragment did not occur after treatment with curcin only. A comparison of the amino acid sequences of curcin, ricin A-chain and trichosanthin revealed that there were relatively high similarities among them. The percentages of homology between curcin and ricin A chain, between curcin and trichosanthin were found to be 54 % and 57 % respectively. Especially, the conserved residues forming the active sites of the A chain of ricin and trichosanthin occurred in curcin. CONCLUSION: Curcin has an obvious antitumor effect and its mechanisms are related to the N-glycosidase activity.
Authors:
Juan Lin; Fang Yan; Lin Tang; Fang Chen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  24     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-05     Completed Date:  2004-12-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  241-6     Citation Subset:  IM    
Affiliation:
College of Life Science, Sichuan University, Chengdu 610064, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antineoplastic Agents, Phytogenic / isolation & purification,  pharmacology*
Humans
Jatropha* / chemistry
Mice
Molecular Sequence Data
N-Glycosyl Hydrolases / metabolism
Plant Proteins / genetics,  isolation & purification,  pharmacology*
Plants, Medicinal / chemistry
Protein Synthesis Inhibitors / pharmacology
RNA, Ribosomal / analysis
Rabbits
Reticulocytes / metabolism
Ribosome Inactivating Proteins
Ricin / genetics
Seeds / chemistry
Sequence Alignment
Sequence Homology, Amino Acid
Trichosanthin / genetics
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Plant Proteins; 0/Protein Synthesis Inhibitors; 0/RNA, Ribosomal; 60318-52-7/Trichosanthin; 9009-86-3/Ricin; EC 3.2.2.-/N-Glycosyl Hydrolases; EC 3.2.2.22/Ribosome Inactivating Proteins

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