| Antitumor effects of curcin from seeds of Jatropha curcas. | |
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MedLine Citation:
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PMID: 12617773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To study the antitumor effects of curcin from Jatropha curcas. METHODS: Antitumor activity of curcin was tested by MTT assay. The N-glycosidase activity of curcin was determined by characterization of R-fragment in gel. A cell-free system, rabbit reticulocyte lysate, was introduced to quantify the inhibitory activity of curcin on protein biosynthesis. RESULTS: The curcin had a powerful inhibitory action upon protein synthesis in reticulocyte lysate with an IC50 (95 % confidence limits) value of 0.19 (0.11-0.27) nmol/L. The IC50 (95 % confidence limits) of curcin on SGC-7901, Sp2/0, and human hepatoma was 0.23 (0.15-0.32) mg/L, 0.66 (0.35-0.97) mg/L, 3.16 (2.74-3.58) mg/L, respectively. Curcin was found to have no toxic to Hela cells and normal cells (MRC). After the rRNA of ribosome was treated with curcin and aniline at acidic condition, a cleaved R-fragment of approximately 450 nt appeared, but this fragment did not occur after treatment with curcin only. A comparison of the amino acid sequences of curcin, ricin A-chain and trichosanthin revealed that there were relatively high similarities among them. The percentages of homology between curcin and ricin A chain, between curcin and trichosanthin were found to be 54 % and 57 % respectively. Especially, the conserved residues forming the active sites of the A chain of ricin and trichosanthin occurred in curcin. CONCLUSION: Curcin has an obvious antitumor effect and its mechanisms are related to the N-glycosidase activity. |
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Authors:
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Juan Lin; Fang Yan; Lin Tang; Fang Chen |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 24 ISSN: 1671-4083 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-05 Completed Date: 2004-12-14 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: China |
Other Details:
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Languages: eng Pagination: 241-6 Citation Subset: IM |
Affiliation:
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College of Life Science, Sichuan University, Chengdu 610064, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antineoplastic Agents, Phytogenic / isolation & purification, pharmacology* Humans Jatropha* / chemistry Mice Molecular Sequence Data N-Glycosyl Hydrolases / metabolism Plant Proteins / genetics, isolation & purification, pharmacology* Plants, Medicinal / chemistry Protein Synthesis Inhibitors / pharmacology RNA, Ribosomal / analysis Rabbits Reticulocytes / metabolism Ribosome Inactivating Proteins Ricin / genetics Seeds / chemistry Sequence Alignment Sequence Homology, Amino Acid Trichosanthin / genetics Tumor Cells, Cultured / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Plant Proteins; 0/Protein Synthesis Inhibitors; 0/RNA, Ribosomal; 60318-52-7/Trichosanthin; 9009-86-3/Ricin; EC 3.2.2.-/N-Glycosyl Hydrolases; EC 3.2.2.22/Ribosome Inactivating Proteins |
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