Document Detail


Antitumor activity of alkaloids derived from Amaryllidaceae species.
MedLine Citation:
PMID:  19368123     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of the present study was to investigate the anticancer properties of five alkaloids isolated from Amaryllidaceae, including the inhibitory effect on P-glycoprotein (P-gp) and the apoptosis-inducing capacity. The tested alkaloids were evaluated for their multidrug resistance (MDR)-reversing activity on human MDR1-gene-transfected L5178 mouse lymphoma cells, using the rhodamine-123 (Rh-123) assay. Trisphaeridine and pretazettine increased the intracellular Rh-123 concentration 30- and 50-fold, respectively, as compared to the non-treated cells, and 2-O-acetyllycorine and trisphaeridine were demonstrated by means of the checkerboard method to enhance the antiproliferative activity of doxorubicin on L5178 MDR mouse lymphoma cells. The MTT assay revealed that pretazettine, trisphaeridine and 2-O-acetyllycorine displayed excellent antiproliferative effects on both the human and the mouse cell lines. The apoptosis-inducing activities of selected agents (2-O-acetyllycorine and trisphaeridine) were measured via acridine orange and ethidium bromide dual staining and flow cytometry of the subG1 population.
Authors:
István Zupkó; Borbála Réthy; Judit Hohmann; Joseph Molnár; Imre Ocsovszki; George Falkay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  23     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    2009 Jan-Feb
Date Detail:
Created Date:  2009-04-16     Completed Date:  2009-05-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  41-8     Citation Subset:  IM    
Affiliation:
Department of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, Eötvös u. 6, Hungary. zupko@pharm.u-szeged.hu
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MeSH Terms
Descriptor/Qualifier:
Amaryllidaceae Alkaloids / pharmacology*
Animals
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
DNA Fragmentation
Dioxoles / pharmacology
Doxorubicin / pharmacology
Drug Combinations
Drug Screening Assays, Antitumor
Drug Synergism
Humans
Leukemia L5178 / drug therapy*,  metabolism,  pathology
Liliaceae / chemistry*
Mice
P-Glycoprotein / metabolism
Phenanthridines / pharmacology
Plant Extracts / pharmacology
Rhodamine 123 / metabolism
Transfection
Chemical
Reg. No./Substance:
0/Amaryllidaceae Alkaloids; 0/Antineoplastic Agents; 0/Dioxoles; 0/Drug Combinations; 0/P-Glycoprotein; 0/Phenanthridines; 0/Plant Extracts; 17322-84-8/pretazettine; 224-11-3/trispheridine; 23214-92-8/Doxorubicin; 62669-70-9/Rhodamine 123

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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