| Antitumor activity of alkaloids derived from Amaryllidaceae species. | |
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MedLine Citation:
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PMID: 19368123 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to investigate the anticancer properties of five alkaloids isolated from Amaryllidaceae, including the inhibitory effect on P-glycoprotein (P-gp) and the apoptosis-inducing capacity. The tested alkaloids were evaluated for their multidrug resistance (MDR)-reversing activity on human MDR1-gene-transfected L5178 mouse lymphoma cells, using the rhodamine-123 (Rh-123) assay. Trisphaeridine and pretazettine increased the intracellular Rh-123 concentration 30- and 50-fold, respectively, as compared to the non-treated cells, and 2-O-acetyllycorine and trisphaeridine were demonstrated by means of the checkerboard method to enhance the antiproliferative activity of doxorubicin on L5178 MDR mouse lymphoma cells. The MTT assay revealed that pretazettine, trisphaeridine and 2-O-acetyllycorine displayed excellent antiproliferative effects on both the human and the mouse cell lines. The apoptosis-inducing activities of selected agents (2-O-acetyllycorine and trisphaeridine) were measured via acridine orange and ethidium bromide dual staining and flow cytometry of the subG1 population. |
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Authors:
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István Zupkó; Borbála Réthy; Judit Hohmann; Joseph Molnár; Imre Ocsovszki; George Falkay |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: In vivo (Athens, Greece) Volume: 23 ISSN: 0258-851X ISO Abbreviation: In Vivo Publication Date: 2009 Jan-Feb |
Date Detail:
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Created Date: 2009-04-16 Completed Date: 2009-05-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806809 Medline TA: In Vivo Country: Greece |
Other Details:
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Languages: eng Pagination: 41-8 Citation Subset: IM |
Affiliation:
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Department of Pharmacodynamics and Biopharmacy, University of Szeged, H-6720 Szeged, Eötvös u. 6, Hungary. zupko@pharm.u-szeged.hu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amaryllidaceae Alkaloids
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pharmacology* Animals Antineoplastic Agents / pharmacology* Apoptosis / drug effects Cell Line, Tumor Cell Proliferation / drug effects DNA Fragmentation Dioxoles / pharmacology Doxorubicin / pharmacology Drug Combinations Drug Screening Assays, Antitumor Drug Synergism Humans Leukemia L5178 / drug therapy*, metabolism, pathology Liliaceae / chemistry* Mice P-Glycoprotein / metabolism Phenanthridines / pharmacology Plant Extracts / pharmacology Rhodamine 123 / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/Amaryllidaceae Alkaloids; 0/Antineoplastic Agents; 0/Dioxoles; 0/Drug Combinations; 0/P-Glycoprotein; 0/Phenanthridines; 0/Plant Extracts; 17322-84-8/pretazettine; 224-11-3/trispheridine; 23214-92-8/Doxorubicin; 62669-70-9/Rhodamine 123 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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