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The Antitumor Activity of Antrodia camphorata in Melanoma Cells: Modulation of Wnt/β-Catenin Signaling Pathways.
MedLine Citation:
PMID:  23049605     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicine. The aim of this study was to investigate whether a fermented culture broth of AC could inhibit melanoma proliferation and progression via suppression of the Wnt/β-catenin signaling pathway. In this study, we observed that AC treatment resulted in decreased cell viability and disturbed Wnt/β-catenin cascade in B16F10 and/or B16F1 melanoma cells. This result was accompanied by a decrease in the expression of Wnt/β-catenin transcriptional targets, including c-Myc and survivin. Furthermore, treatment of melanoma cells with AC resulted in a significant increase in apoptosis, which was associated with DNA fragmentation, cytochrome c release, caspase-9 and -3 activation, PARP degradation, Bcl-2/Bax dysregulation, and p53 expression. We also observed that AC caused G(1) phase arrest mediated by a downregulation of cyclin D1 and CDK4 and increased p21 and p27 expression. In addition, we demonstrated that non- and subcytotoxic concentrations of AC markedly inhibited migration and invasion of highly metastatic B16F10 cells. The antimetastatic effect of AC was further confirmed by reductions in the levels of MMP-2, MMP-9, and VEGF expression. These results suggest that Antrodia camphorata may exert antitumor activity by downregulating the Wnt/β-catenin pathways.
Authors:
You-Cheng Hseu; Hsiao-Tung Tsou; K J Senthil Kumar; Kai-Yuan Lin; Hsueh-Wei Chang; Hsin-Ling Yang
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Publication Detail:
Type:  Journal Article     Date:  2012-09-25
Journal Detail:
Title:  Evidence-based complementary and alternative medicine : eCAM     Volume:  2012     ISSN:  1741-4288     ISO Abbreviation:  Evid Based Complement Alternat Med     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-10     Completed Date:  2012-10-11     Revised Date:  2013-04-08    
Medline Journal Info:
Nlm Unique ID:  101215021     Medline TA:  Evid Based Complement Alternat Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  197309     Citation Subset:  -    
Affiliation:
Department of Cosmeceutics, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
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