Document Detail


Antitrypanosomal compounds from the essential oil and extracts of Keetia leucantha leaves with inhibitor activity on Trypanosoma brucei glyceraldehyde-3-phosphate dehydrogenase.
MedLine Citation:
PMID:  23312849     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Keetia leucantha is a West African tree used in traditional medicine to treat several diseases among which parasitic infections. The dichloromethane extract of leaves was previously shown to possess growth-inhibitory activities on Plasmodium falciparum, Trypanosoma brucei brucei and Leishmania mexicana mexicana with low or no cytotoxicity (>100μg/ml on human normal fibroblasts) (Bero et al. 2009, 2011). In continuation of our investigations on the antitrypanosomal compounds from this dichloromethane extract, we analyzed by GC-FID and GC-MS the essential oil of its leaves obtained by hydrodistillation and the major triterpenic acids in this extract by LC-MS. Twenty-seven compounds were identified in the oil whose percentages were calculated using the normalization method. The essential oil, seven of its constituents and the three triterpenic acids were evaluated for their antitrypanosomal activity on Trypanosoma brucei brucei bloodstream forms (Tbb BSF) and procyclic forms (Tbb PF) to identify an activity on the glycolytic process of trypanosomes. The oil showed an IC(50) of 20.9μg/ml on Tbb BSF and no activity was observed on Tbb PF. The best antitrypanosomal activity was observed for ursolic acid with IC(50) of 2.5 and 6.5μg/ml respectively on Tbb BSF and Tbb PF. The inhibitory activity on a glycolytic enzyme of T. brucei, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), was also evaluated for betulinic acid, olenaolic acid, ursolic acid, phytol, α-ionone and β-ionone. The three triterpenic acids and β-ionone showed inhibitory activities on GAPDH with oleanolic acid being the most active with an inhibition of 72.63% at 20μg/ml. This paper reports for the first time the composition and antitrypanosomal activity of the essential oil of Keetia leucantha. Several of its constituents and three triterpenic acids present in the dichloromethane leaves extract showed a higher antitrypanosomal activity on bloodstream forms of Tbb as compared to procyclic forms, namely geranyl acetone, phytol, α-ionone, β-ionone, ursolic acid, oleanolic acid and betulinic acid. The four last compounds were proven to be inhibitors of trypanosomal GAPDH, which may in part explain these antitrypanosomal activities.
Authors:
J Bero; C Beaufay; V Hannaert; M-F Hérent; P A Michels; J Quetin-Leclercq
Related Documents :
24497059 - Inhibitors from carob (ceratonia siliqua l.) : iii. comparisons with abscisic acid.
24026929 - Microbial communities and chemical changes during fermentation of sugary brazilian kefir.
17885679 - The integration of acetic acid iontophoresis, orthotic therapy and physical rehabilitat...
23376199 - Simultaneous acetic acid separation and monosaccharide concentration by reverse osmosis.
19747599 - Biodiversity, ecological determinants, and metabolic exploitation of sourdough microbiota.
9553799 - Characterization of bifidobacterium strains for use in soymilk fermentation.
19137319 - Lake char (salvelinus namaycush) olfactory neurons are highly sensitive and specific to...
10320719 - Effects of hyperosmolarity and ion substitutions on amino acid efflux from the ischemic...
1248929 - Studies on trypsin inhbitors. part ii. synthesis of the protected tetrapeptide (sequenc...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Phytomedicine : international journal of phytotherapy and phytopharmacology     Volume:  -     ISSN:  1618-095X     ISO Abbreviation:  Phytomedicine     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438794     Medline TA:  Phytomedicine     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier GmbH. All rights reserved.
Affiliation:
Université catholique de Louvain, Louvain Drug Research Institute, Pharmacognosy Research Group, Avenue E. Mounier, B1.72.03, B-1200 Brussels, Belgium. Electronic address: joanne.bero@uclouvain.be.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Construction, expression, purification and antigenicity of recombinant Campylobacter jejuni flagella...
Next Document:  VZV multifocal vasculopathy with ischemic optic neuropathy, acute retinal necrosis and temporal arte...