| Antithrombotic effect of a monoclonal antibody against tissue factor in a rabbit model of platelet-mediated arterial thrombosis. | |
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MedLine Citation:
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PMID: 1637793 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activation of the coagulation system by contact of circulating blood with tissue factor, a component of the extrinsic blood coagulation pathway that is produced in the vessel wall, may represent a pathway for the initiation of thrombosis in atherosclerotic vessels. This hypothesis was tested in vivo in a rabbit femoral artery eversion (inside-out) graft model, in which the adventitia, with its tissue factor, was exposed to circulating blood. Intra-arterial infusion of a neutralizing monoclonal antibody against tissue factor (D3) at a rate of 12 mg/kg over 15 minutes prevented thrombosis of a 7-8-mm eversion graft within a 2-hour observation period in four of five rabbits, whereas with a control antibody infusion (MA-15C5), occlusion occurred within 2 hours in five of six rabbits. In vitro immersion before reinsertion of the arterial segment in a solution containing 2 mg/ml of the control antibody for 30 minutes was associated with occlusion in all six rabbits, whereas pretreatment with D3 was associated with persistent patency in three of nine rabbits. Stepwise logistic-regression analysis of the results with perfusion status as the dependent variable and type of antibody (D3 or MA-15C5), application method (infusion versus immersion), and graft segment length as independent variables yielded a significant difference in frequency of occlusion with the two antibodies (p = 0.016). It is concluded that exposure of tissue factor to flowing blood may constitute a trigger mechanism for platelet-mediated arterial thrombosis. |
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Authors:
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I K Jang; H K Gold; R C Leinbach; J T Fallon; D Collen; J N Wilcox |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Arteriosclerosis and thrombosis : a journal of vascular biology / American Heart Association Volume: 12 ISSN: 1049-8834 ISO Abbreviation: Arterioscler. Thromb. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-09-01 Completed Date: 1992-09-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9101388 Medline TA: Arterioscler Thromb Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 948-54 Citation Subset: IM |
Affiliation:
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Cardiac Unit, Massachusetts General Hospital, Boston 02114. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / therapeutic use* Blood Platelets / physiology* Rabbits Thromboplastin / immunology, physiology* Thrombosis / etiology, pathology, therapy* |
| Grant Support | |
ID/Acronym/Agency:
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HL-26205/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 9035-58-9/Thromboplastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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