Document Detail


Antisperm antibodies in prepubertal boys and their reactivity with antigenic determinants on differentiated spermatozoa.
MedLine Citation:
PMID:  9764368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PROBLEM: Antisperm antibodies induced in prepubertal boys with testicular failures were characterized by using four techniques of antibody detection. The reactivity of circulating antisperm antibodies in prepubertal boys and the reactivity of antibodies in sera samples of adult fertile and infertile males were compared against the same sperm antigenic pools (live or fixed spermatozoa, or sperm antigenic extracts). METHOD OF STUDY: The incidence of antisperm antibodies in sera samples of 69 prepubertal boys with testicular failures and 21 samples obtained from adult, male individuals was assessed by indirect immunobead binding test (IDIBT), flow cytometry measurement, enzyme-linked immunosorbent assay, and Western blotting. Immunoblot analysis was performed by using sperm extracts of glycosylated and deglycosylated solubilized membrane antigens. RESULTS: Sera samples were studied in a group composed of healthy prepubertal boys (n = 7) and prepubertal boys with testicular failures (n = 69). Applied tests of antibody detection revealed striking differences in a group of boys with testicular pathology. With IDIBT, 7% of the sera samples were found positive, whereas with flow cytometry measurement, 48% of the sera samples were positive. Immunosorbent assay (fixed sperm) indicated 32% positive cases in the same group. The sera samples were found to be positive in 65% of immunoblotting reactions with glycosylated antigens and in 70% of immunoblotting reactions with deglycosylated antigens. All applied detection assays were clearly negative on sera samples from fertile, adult males. Western immunoblotting indicated an immunodominant antigenic determinant of 58 kDa. CONCLUSIONS: Tests of antibody detection with the use of live sperm (IDIBT and flow cytometry measurements) presented low sensitivity (8% and 48%, respectively) in a group of prepubertal boys. This observation underlines the difficulties in assigning the prospective prognosis of future fertility status in prepubertal boys with antisperm antibodies.
Authors:
A Domagała; M Kamieniczna; D Kowalczyk; M Kurpisz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  40     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-02     Completed Date:  1998-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  223-9     Citation Subset:  IM    
Affiliation:
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Antibodies / blood*,  immunology
Antigen-Antibody Reactions / immunology*
Antigens / blood,  immunology
Cell Differentiation / physiology
Child
Child, Preschool
Epitopes / immunology*
Glycosylation
Humans
Immunoblotting
Male
Puberty / immunology*
Spermatozoa / cytology,  immunology*
Testicular Diseases / blood,  immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens; 0/Epitopes

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