Document Detail

Antisense phosphorothioate oligodeoxynucleic acid for CD10 suppresses liver metastasis of colorectal cancer.
MedLine Citation:
PMID:  19816087     Owner:  NLM     Status:  MEDLINE    
CD10 expression is associated with metastases of colorectal cancer (CRC). In the present study, we examined association of CD10 with liver metastasis of CRC cells to clarify the therapeutic significance of CD10. CD10-positive human colon cancer cell line, HT29 cells showed inhibition of growth, invasion and colony formation by treatment with CD10 antisense S-oligodeoxynucleotide (S-ODN). In the mouse liver metastasis mode, CD10 antisense S-ODN-treated HT29 cells made less embedded cells in the liver than control HT29 cells. Number and size of metastatic foci in nude mice liver were reduced in CD10 antisense S-ODN-treated HT29 cells. Treatment with CD10 antisense S-ODN decreased phosphorylation of ERK1/2 and EGFR in HT29 cells. Intraperitoneal administration of liposome-capsulated CD10 antisense S-ODN inhibited establishment of liver metastasis and growth of established metastasis in nude mice. These findings suggest that CD10 is associated substantially with liver metastasis of CRC cells and might be a molecular target of CRC treatment.
Yi Luo; Kiyomu Fujii; Hitoshi Ohmori; Tomonori Sasahira; Yukiko Moriwaka; Mamoru Isobe; Hiroki Kuniyasu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-02
Journal Detail:
Title:  Pathobiology : journal of immunopathology, molecular and cellular biology     Volume:  76     ISSN:  1423-0291     ISO Abbreviation:  Pathobiology     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-09     Completed Date:  2009-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007504     Medline TA:  Pathobiology     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  267-73     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Department of Molecular Pathology, Nara Medical University, Kashihara, Japan.
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MeSH Terms
Blotting, Western
Colorectal Neoplasms / drug therapy,  metabolism*,  pathology*
HT29 Cells
Liver Neoplasms, Experimental / drug therapy,  metabolism*,  secondary*
Mice, Nude
Neprilysin / metabolism*
Oligodeoxyribonucleotides, Antisense / pharmacology
Phosphorothioate Oligonucleotides / pharmacology
Xenograft Model Antitumor Assays
Reg. No./Substance:
0/Oligodeoxyribonucleotides, Antisense; 0/Phosphorothioate Oligonucleotides; EC

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