Document Detail

Antisense oligonucleotide targeting p53 increased apoptosis of MCF-7 cells induced by ionizing radiation.
MedLine Citation:
PMID:  17049121     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the effect of antisense compounds (AS) targeting human p53 mRNA on radiosensitivity of MCF-7 cells. METHODS: Western blotting and RTPCR were used to analyze the protein content and mRNA level. Additionally, cell proliferation, cell cycle and cell apoptosis were all analyzed in irradiated or sham-irradiated cells. RESULTS: Among the five antisense compounds (AS), AS3 was identified to efficiently inhibit p53 mRNA level and protein content. Interestingly, AS3 transfer has little effect on cell proliferation in DU-145 cells (mutant p53) after ionizing radiation (IR). In contrast, a marked increase of cell apoptosis and growth inhibition were observed in MCF-7 cells (wild-type p53), suggesting that AS3 can increase radiosensitivity of MCF-7 cells. Additionally, it was also observed that the transfection of AS3 decreased the fraction of G1 phase cells, and increased the proportion of S phase cells compared to untreated cells 24 h after IR in MCF-7 cell lines. CONCLUSION: AS3 transfection increases MCF-7 cell apoptosis induced by 5 Gy-radiation, and this mechanism may be closely associated with abrogation of G1 phase arrest.
Li-cheng Dai; Xiang Wang; Xing Yao; Li-shan Min; Fu-chu Qian; Jian-fang He
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  27     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-19     Completed Date:  2007-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  1453-8     Citation Subset:  IM    
Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, China.
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MeSH Terms
Apoptosis / radiation effects*
Breast Neoplasms / metabolism,  pathology
Cell Cycle / genetics,  radiation effects
Cell Line, Tumor
Cell Proliferation / radiation effects
Genes, p53 / genetics*
Oligonucleotides, Antisense / genetics*
Prostatic Neoplasms / metabolism,  pathology
RNA, Messenger / biosynthesis,  genetics
Radiation, Ionizing
Tumor Suppressor Protein p53 / biosynthesis*,  genetics
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53

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