Document Detail


Antisense oligodeoxynucleotides targeting the serine/threonine kinase Pim-2 inhibited proliferation of DU-145 cells.
MedLine Citation:
PMID:  15715935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the effect of antisense oligodeoxynucleotides (ASODN) targeting Pim-2 on cell proliferation of DU-145 cells. METHODS: Three ASODN targeting Pim-2 were designed and synthesized. After transfection with ASODN, cell proliferation was analyzed using an MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] assay. In addition, Pim-2 mRNA, protein levels, and cell cycles were examined. RESULTS: The ASODN designed and synthesized by our laboratory significantly reduced Pim-2 mRNA level and protein content in DU-145 cells. After transfection with ASODN for 48 h, a marked reduction in cell viability was observed in DU-145 cells in a dose-dependent manner. No remarkable apoptosis occurred in cells treated with ASODN compared with control cells. However, it should be noted that G1 phase arrest was clearly observed in ASODN-treated cells. CONCLUSION: ASODN targeting Pim-2 resulted in a marked reduction in DU-145 cell proliferation, and induction of G1 phase cell cycle arrest is one of the important mechanisms for ASODN to reduce cell growth. Moreover, antisense inhibition of Pim-2 expression provides a new promising therapy target for prostate cancer.
Authors:
Jin-ming Dai; Shu-qun Zhang; Wei Zhang; Ru-xian Lin; Zong-zheng Ji; Sheng-qi Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  26     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-17     Completed Date:  2005-10-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  364-8     Citation Subset:  IM    
Affiliation:
Beijing Institute of Radiation Medicine, Beijing 100850, China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects*
G1 Phase
Humans
Male
Oligodeoxyribonucleotides, Antisense / genetics,  pharmacology*
Prostatic Neoplasms / metabolism,  pathology
Protein-Serine-Threonine Kinases / biosynthesis*,  genetics
Proto-Oncogene Proteins / biosynthesis*,  genetics
RNA, Messenger / biosynthesis,  genetics
Transfection*
Chemical
Reg. No./Substance:
0/Oligodeoxyribonucleotides, Antisense; 0/PIM2 protein, human; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; EC 2.7.11.1/Protein-Serine-Threonine Kinases

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