| Antisense oligodeoxynucleotide-conjugated hyaluronic acid/protamine nanocomplexes for intracellular gene inhibition. | |
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MedLine Citation:
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PMID: 17602578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Green fluorescent protein (GFP) antisense oligodeoxynucleotide (ODN) was covalently conjugated to hyaluronic acid (HA) via a reducible disulfide linkage, and the HA-ODN conjugate was complexed with protamine to increase the extent of cellular uptake and enhance the gene inhibition efficiency of GFP expression. The HA-ODN conjugate formed more stable polyelectrolyte complexes with protamine as compared to naked ODN, probably because of its increased charge density. The higher cellular uptake of protamine/HA-ODN complexes than that of protamine/naked ODN complexes was attributed to the formation of more compact nanosized complexes (approximately 200 nm in diameter) in aqueous solution. Protamine/HA-ODN complexes also showed a comparable level of GFP gene inhibition to that of cytotoxic polyethylenimine (PEI)/ODN complexes. Since both HA and protamine are naturally occurring biocompatible materials, the current formulation based on a cleavable conjugation strategy of ODN to HA could be potentially applied as safe and effective nonviral carriers for ODN and siRNA nucleic acid therapeutics. |
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Authors:
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Hyejung Mok; Ji Won Park; Tae Gwan Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-28 |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 18 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2007 Sep-Oct |
Date Detail:
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Created Date: 2007-09-19 Completed Date: 2007-11-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1483-9 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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chemical synthesis,
pharmacology* Disulfides / chemistry Drug Carriers / pharmacology Gene Silencing / drug effects*, physiology Gene Therapy / methods Green Fluorescent Proteins / chemistry Hyaluronic Acid / chemistry* Nanostructures / chemistry* Oligonucleotides, Antisense / chemistry* Protamines / chemistry* RNA, Small Interfering / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Disulfides; 0/Drug Carriers; 0/Oligonucleotides, Antisense; 0/Protamines; 0/RNA, Small Interfering; 147336-22-9/Green Fluorescent Proteins; 9004-61-9/Hyaluronic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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