| Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells. | |
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MedLine Citation:
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PMID: 15511236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glutamine is an essential amino acid in cancer cells and is required for the growth of many other cell types. Glutaminase activity is positively correlated with malignancy in tumours and with growth rate in normal cells. In the present work, Ehrlich ascites tumour cells, and their derivative, 0.28AS-2 cells, expressing antisense glutaminase mRNA, were assayed for apoptosis induced by methotrexate and hydrogen peroxide. It is shown that Ehrlich ascites tumour cells, expressing antisense mRNA for glutaminase, contain lower levels of glutathione than normal ascites cells. In addition, 0.28AS-2 cells contain a higher number of apoptotic cells and are more sensitive to both methotrexate and hydrogen peroxide toxicity than normal cells. Taken together, these results provide insights into the role of glutaminase in apoptosis by demonstrating that the expression of antisense mRNA for glutaminase alters apoptosis and glutathione antioxidant capacity. |
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Authors:
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Jorge Lora; Francisco J Alonso; Juan A Segura; Carolina Lobo; Javier Márquez; José M Matés |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 271 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-10-29 Completed Date: 2004-12-17 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: Germany |
Other Details:
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Languages: eng Pagination: 4298-306 Citation Subset: IM |
Affiliation:
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Departamento de Biología Molecular y Bioquímica, Laboratorio de Química de Proteínas, Facultad de Ciencias, Universidad de Málaga, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / metabolism* Apoptosis* Carcinoma, Ehrlich Tumor / genetics*, pathology* Caspase 3 Caspases / metabolism Cell Line, Tumor Cell Membrane / metabolism Cell Proliferation Cell Separation Cell Survival DNA / chemistry DNA Fragmentation Dose-Response Relationship, Drug Electrophoresis, Agar Gel Flow Cytometry Glutaminase / antagonists & inhibitors*, genetics* Glutathione / metabolism Glutathione Reductase / metabolism Hydrogen Peroxide / pharmacology Methotrexate / pharmacology Mice Oligonucleotides, Antisense / chemistry* Phosphatidylserines / chemistry RNA, Messenger / metabolism Rats Reactive Oxygen Species Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Oligonucleotides, Antisense; 0/Phosphatidylserines; 0/RNA, Messenger; 0/Reactive Oxygen Species; 59-05-2/Methotrexate; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; 9007-49-2/DNA; EC 1.8.1.7/Glutathione Reductase; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases; EC 3.5.1.2/Glutaminase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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