Document Detail

Antisense glutaminase inhibition decreases glutathione antioxidant capacity and increases apoptosis in Ehrlich ascitic tumour cells.
MedLine Citation:
PMID:  15511236     Owner:  NLM     Status:  MEDLINE    
Glutamine is an essential amino acid in cancer cells and is required for the growth of many other cell types. Glutaminase activity is positively correlated with malignancy in tumours and with growth rate in normal cells. In the present work, Ehrlich ascites tumour cells, and their derivative, 0.28AS-2 cells, expressing antisense glutaminase mRNA, were assayed for apoptosis induced by methotrexate and hydrogen peroxide. It is shown that Ehrlich ascites tumour cells, expressing antisense mRNA for glutaminase, contain lower levels of glutathione than normal ascites cells. In addition, 0.28AS-2 cells contain a higher number of apoptotic cells and are more sensitive to both methotrexate and hydrogen peroxide toxicity than normal cells. Taken together, these results provide insights into the role of glutaminase in apoptosis by demonstrating that the expression of antisense mRNA for glutaminase alters apoptosis and glutathione antioxidant capacity.
Jorge Lora; Francisco J Alonso; Juan A Segura; Carolina Lobo; Javier Márquez; José M Matés
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  271     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-10-29     Completed Date:  2004-12-17     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  4298-306     Citation Subset:  IM    
Departamento de Biología Molecular y Bioquímica, Laboratorio de Química de Proteínas, Facultad de Ciencias, Universidad de Málaga, Spain.
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MeSH Terms
Antioxidants / metabolism*
Carcinoma, Ehrlich Tumor / genetics*,  pathology*
Caspase 3
Caspases / metabolism
Cell Line, Tumor
Cell Membrane / metabolism
Cell Proliferation
Cell Separation
Cell Survival
DNA / chemistry
DNA Fragmentation
Dose-Response Relationship, Drug
Electrophoresis, Agar Gel
Flow Cytometry
Glutaminase / antagonists & inhibitors*,  genetics*
Glutathione / metabolism
Glutathione Reductase / metabolism
Hydrogen Peroxide / pharmacology
Methotrexate / pharmacology
Oligonucleotides, Antisense / chemistry*
Phosphatidylserines / chemistry
RNA, Messenger / metabolism
Reactive Oxygen Species
Time Factors
Reg. No./Substance:
0/Antioxidants; 0/Oligonucleotides, Antisense; 0/Phosphatidylserines; 0/RNA, Messenger; 0/Reactive Oxygen Species; 59-05-2/Methotrexate; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; 9007-49-2/DNA; EC Reductase; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases; EC

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